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| 兔骨髓间充质干细胞定向诱导分化为脂肪细胞及成骨细胞的研究 | |
| 引用本文： | 李胜富,黄定强,卢晓风,刘瑾,孙明菡,李幼平,程惊秋,步宏,梁传余. 兔骨髓间充质干细胞定向诱导分化为脂肪细胞及成骨细胞的研究[J]. 生物医学工程学杂志, 2003, 20(2): 209-213 |
| 作者姓名： | 李胜富 黄定强 卢晓风 刘瑾 孙明菡 李幼平 程惊秋 步宏 梁传余 |
| 作者单位： | 1. 四川大学华西医院卫生部移植工程与移植免疫重点实验室,成都,610041 2. 四川大学华西医院耳鼻喉科,成都,610041 3. 四川大学华西医院病理科,成都,610041 |
| 基金项目： | 美国纽约中华医学基金会资助(CMB综合发展项目 -9663 6) |
| 摘 要： | 用密度梯度离心和贴壁法分离和纯化兔骨髓间充干细胞，建立诱导兔MSCs向脂肪细胞及成骨细胞表型转化的方法及条件。在成脂诱导剂或成骨诱导剂作用下，对原代和第2代兔MSCs进行成脂和成骨诱导培养，并鉴定成脂及成骨表型。结果表明：原代及第2代兔MSCs均有一定的成脂、成骨能力，且第2代细胞的分化能力较原代低。在诱导培养条件下，原代及第2代兔MSCs均能分化，成脂诱导21d，75％的兔MSCs转化为脂肪细胞；成骨诱导21d，75％的兔MSCs转化为成骨细胞。兔MSCs在适当的诱导条件下可快速分化为脂肪细胞或成骨细胞。 |
| 关 键 词： | 骨髓间充质干细胞 定向诱导 细胞分化 脂肪细胞 成骨细胞 组织工程 |
Study on Rabbit Mesenchymal Stem Cells Differentiation to the Adipogenic or Osteogenic Lineage in vitro |
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| Li Shengfu Huang Dingqiang Lu Xiaofeng Liu Jin Sun Minghan Li Youping Cheng Jingqiu Bu Hong Liang Chuanyu. Study on Rabbit Mesenchymal Stem Cells Differentiation to the Adipogenic or Osteogenic Lineage in vitro[J]. Journal of biomedical engineering, 2003, 20(2): 209-213 | |
| Authors: | Li Shengfu Huang Dingqiang Lu Xiaofeng Liu Jin Sun Minghan Li Youping Cheng Jingqiu Bu Hong Liang Chuanyu |
| Affiliation: | Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West China Hospital of Sichuan University, Chengdu 610041. |
| Abstract: | Rabbit bone marrow derived mesenchymal stem cells(MSCs)are multipotent.We studied the adipogenic and osteogenic differentiation poteint using adipogenic supplement (AS) or osteogenic supplement (OS) in vitro. Specific markers of this induced adipogenic and osteogenic lineage were identified. The findings showed that the rabbit MSCs are capable of differentiating into adipogenic and osteogenic lineages spontaneously. On the 21th day, approximately 75% rabbit MSCs were induced to adipogenic or osteogenic cells in medium containing AS or OS, respectively. These results demonstrated that the differentiation of MSCs could be regulated in vitro. The underlying molecular mechanisms of adipogenic or osteogenic differentiation await elucidation. |
| Keywords: | Mesenchymal stem cells(MSCs) Differentiation Adipocyte Osteoblast |
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