血管内皮生长因子和核转录因子p65在多形性腺瘤中的表达及其意义

目的 探讨血管内皮生长因子(VEGF)和核转录因子(NF-κB)p65在不同分型的多形性腺瘤中的表达及意义.方法 涎腺多形性腺瘤患者60例,其中细胞丰富型31例,基质丰富型29例,取瘤旁正常涎腺组织30份,应用免疫组织化学技术检测组织中VEGF、NF-κBp65的表达.结果 (1)VEGF表达主要以肿瘤性上皮组织较多,多为实性上皮条索、腺管样结构区域,染色强度不尽相同.(2)多形性腺瘤组织中NF-κB p65阳性表达主要见于腺泡上皮和导管上皮,呈强阳性染色;间质成分中血管上皮呈弱阳性表达、纤维结缔组织呈弱阳性表达;黏液样组织和软骨样组织未见明显阳性表达.(3)VEGF在细胞丰富型腺瘤中平均吸光度(MA)值为955.67±305.79,基质丰富型MA值为149.13±60.85,正常对照组MA值为53.46±9.66,两两比较差异均有统计学意义(P均＜0.05).(4)NF-κB p65在细胞丰富型腺瘤MA值为529.80±164.81,基质丰富型为43.40±5.46,正常对照组为6.84±1.91,两两比较差异均有统计学意义(P均＜0.05).<5)涎腺多形性腺瘤中NF-κB p65与VEGF表达呈正相关(r=0.854,P＜0.05).结论 (1)涎腺多形性腺瘤新生血管形成能力及细胞增殖活性随着瘤体中细胞成分的增多而逐渐升高,细胞丰富型多形性腺瘤较基质丰富型多形性腺瘤具有更易恶变的倾向.(2)NF-κB p65可能是通过作用于VEGF来实现对新生血管的调控作用.

Abstract：

Objective To investigate the expression and significance of VEGF and NF-κB/p65 in the two subtypes of pleomorphic adenoma. Methods Immunohistochemistry was applied to detect the expression of VEGF and NF-κB/p65 in pleomorphic adenoma of 60 patients including 31 cases of cell-rich and 29 cases of stroma-rich as well as normal salivary gland tissues of 30 cases from adjacent tumor. Results VEGF positive staining was mainly found in tumor epithelia,while NF-κB/p65 positive was detected in gland alveolus cell and ductal epithelia. The Mean optical density( MA ) values of VEGF were 955.67 ± 305.79,149. 13 ± 60. 85 and 53.46 ± 9. 66, respectively, in cell-rich adenoma, stroma-rich adenoma and normal control. The difference in VEGF expression between the groups was significant (Ps ＜ 0. 05 ) . The MA values of NF-κB/p65 were 529. 80 ± 164. 81,43.40 ±5.46 and 6. 84 ± 1.91 ,respectively,in three groups mentioned above. The difference in NF-κB/p65 expression between the groups was significant ( Ps ＜ 0. 05 ). In pleomorphic adenoma, the expression level of NF-κB/p65 was positively correlated with VEGF. Conclusion ( 1 ) The angiogenesis and proliferation potential of carcinomas increased with the cell component in pleomorphic adenoma. Stroma-poor adenoma is more frequently subjected to malignant transformation than stroma-rich adenoma. (2) NF-κB/p65 may have effects on angiogenesis by activating VEGF. Detecting the expression of VEGF and NF-κB/p65 may be helpful to predict the biological behavior of pleomorphic adenoma and prognosis of the patients, which couldprovide useful information for future targeted therapy of pleomorphic adenoma.