血浆内皮素和一氧化氮以及血管内皮生长因子对2型糖尿病视网膜病变的影响

目的 探讨2型糖尿病视网膜病变(DR)患者血浆中内皮素(ET)、一氧化氮(NO)和血管内皮生长因子(VEGF)的变化.方法 选择2型糖尿病患者80例,其中有视网膜病变53例(DR组),无视网膜病变27例(NDR组),另设健康对照者30名作为对照组.采用酶联免疫吸附双抗体夹心法测定其血浆ET和VEGF,硝酸还原法测定NO,并检测空腹血糖、餐后2 h血糖、糖化血红蛋白、胆固醇、甘油三酯.结果 DR组患者血浆ET为(80.68±13.57)mg/L,高于NDR组(65.33±11.24)mg/L]及对照组(45.25±9.06)mg/L],差异均有统计学意义(P均＜0.01);DR组NO为(69.82±14.89)μmol/L,明显高于对照组(37.85±9.11)μmol/L],但低于NDR组(77.52±18.56)μmol/L],差异均有统计学意义(P均＜0.05);DR组VEGF(110.52±25.65)μg/L]高于对照组(82.42±18.47)μg/L]及NDR组(97.55±25.61)μg/L],差异均有统计学意义(P均＜0.05).结论 ET、NO和VEGF可能参与了2型糖尿病视网膜病变的发生与发展.

Abstract：

Objective To study the changes of endothelin,nitric oxide and vascular endothelial growth factor level in patients with type 2 diabetic retinopathy (DR). Methods Eighty diabetes patients (53 with diabetic retinopathy and 27 without). Another 30 healthy volunteers were recruited as control. Plasma ET and VEGF levels were measured by enzyme-linked immunosorbent assay. NO levels were measured by nitrate reductase method. Results Plasma levels of ET were higher in patients with type 2 diabetes with DR (DR)(80. 68 ± 13.57) mg/L than (65. 33 ± 11.24) mg/L, (45.25 ±9. 06) mg/L, in control and in type 2 diabetes patients without DR (NDR) (Ps ＜ 0, 01 ). Plasna levels of NO in DR group (69. 82 ± 14. 89) μmol/L were higher than (37. 85 ±-9. 11 ) μmol'L, in control group,but lower than (77.52 ±± 18.56) μmol/L in NDR group (Ps ＜ 0. 05 ). Plasma levels of VEGF ( 110. 52 ± 25.65 ) μg/L in DR were significantly increased compared with control (82.42 ± 18. 47 ) μg/L, and NDR(97.55 ±25.61)μg/L, (Ps ＜0.05).Conclusion ET, NO and VEGF may be involved in the pathogenesis of type 2 diabetic retinopathy.

The effects of endothelin, nitric oxide and vascular endothelial growth factor levels on the patients with with type 2 diabetic retinopathy

Objective To study the changes of endothelin,nitric oxide and vascular endothelial growth factor level in patients with type 2 diabetic retinopathy (DR). Methods Eighty diabetes patients (53 with diabetic retinopathy and 27 without). Another 30 healthy volunteers were recruited as control. Plasma ET and VEGF levels were measured by enzyme-linked immunosorbent assay. NO levels were measured by nitrate reductase method. Results Plasma levels of ET were higher in patients with type 2 diabetes with DR (DR)(80. 68 ± 13.57) mg/L than (65. 33 ± 11.24) mg/L, (45.25 ±9. 06) mg/L, in control and in type 2 diabetes patients without DR (NDR) (Ps ＜ 0, 01 ). Plasna levels of NO in DR group (69. 82 ± 14. 89) μmol/L were higher than (37. 85 ±-9. 11 ) μmol'L, in control group,but lower than (77.52 ±± 18.56) μmol/L in NDR group (Ps ＜ 0. 05 ). Plasma levels of VEGF ( 110. 52 ± 25.65 ) μg/L in DR were significantly increased compared with control (82.42 ± 18. 47 ) μg/L, and NDR(97.55 ±25.61)μg/L, (Ps ＜0.05).Conclusion ET, NO and VEGF may be involved in the pathogenesis of type 2 diabetic retinopathy.