胎儿脐血间充质干细胞治疗大鼠急性心肌梗死

背景：胎儿脐血间充质干细胞对病变心肌有修复和再生能力，胎儿脐血间充质干细胞移植是治疗心肌梗死的一种新途径。 目的：探讨胎儿脐血间充质干细胞移植治疗大鼠心肌梗死的效果。 方法：选取32只大鼠结扎左冠状动脉前降支制作心肌梗死动物模型，随机等分为移植组和梗死组，从胎儿脐血中分离培养脐血间充质干细胞，制备脐血间充质干细胞悬液，对移植组大鼠进行脐血间充质干细胞移植。 结果与结论：胎儿脐血间充质干细胞在体外可以被成功分离培养；与梗死组相比，移植组大鼠心肌梗死边缘区的微血管密度、左室收缩末压、左室内压最大上升和下降速率显著增加(P < 0.05)，左室舒张末压显著下降(P < 0.05)，心电图情况稍有好转。表明胎儿脐血间充质干细胞治疗心肌梗死大鼠可以促进心肌血管再生，改善心脏功能。中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Transplantation of human umbilical cord blood-derived mesenchymal stem cells for treatment of acute myocardial infarction in rats

BACKGROUND:Human umbilical cord blood-derived mesenchymal stem cells are able to repair and regenerate the injured myocardium, which is a new therapy for myocardial infarction via transplantation. OBJECTIVE:To explore the therapeutic efficacy of intracoronary injection of human umbilical cord blood-derived mesenchymal stem cells on acute myocardial infarction in rats. METHODS:Thirty-two rats were selected to make animal models of ligation of the left anterior descending coronary, and then model rats were randomized equally to transplantation group and model group. Human umbilical cord blood-derived mesenchymal stem cells were isolated and prepared into cell suspension. Rats in the transplantation group were subjected to transplantation of human umbilical cord blood-derived mesenchymal stem cells. RESULTS AND CONCLUSION:Human umbilical cord blood-derived mesenchymal stem cells were successfully isolated and cultured in vitro. Compared with the model group, the microvessel density, left ventricular end-systolic pressure and ±dp/dtmax were significantly increased in the transplantation group (P < 0.05), while the left ventricular end-diastolic pressure was decreased dramatically (P < 0.05). Electrocardiography findings showed that the heart function of rats in the transplantation group was improved slightly. These findings indicate that human umbilical cord blood-derived mesenchymal stem cells can promote myocardial angiogenesis and improve heart function of rats with myocardial infarction.