原位肝移植模型的建立技巧与改进

背景：目前肝移植是治愈终末期肝病惟一的方法，但是其移植后的并发症仍然是影响移植患者长期存活的重要因素，因此有必要建立稳定的动物移植模型。目的：建立原位肝移植模型大鼠。方法：应用乙醚吸入法对SD大鼠204只行麻醉，2-4 ℃林格液经腹主动脉进行单路灌注，灌注前不翻动肝脏，以减少肝脏热缺血，灌注完毕后沿膈环下切断肝上下腔静脉，修整时不需再修整，以免损伤腔静脉。取下供体肝脏，置入4 ℃淋格液中保存，切下受体肝脏，采用改良的二袖套法行同种异体原位肝移植。移植完毕后，大鼠可自行翻身饮水，存活超过3 d，视为移植成功。结果与结论：204只大鼠共行102次肝移植，最终86例移植后存活超过3 d，移植成功率为84%。结果证实，实验通过改进技术成功建立了原位肝移植模型大鼠。中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Techniques and improvements of establishing orthotopic liver transplantation rat models

BACKGROUND:At present, liver transplantation is the only way to cure end-stage liver disease, but the complications after transplantation is still an important factor of affecting the long-term survival of patients who received orthotopic liver transplantation, therefore it is necessary to establish a stable animal transplantation model. OBJECTIVE:To establish rat models of orthotopic liver transplantation. METHODS:After inhalation anesthesia with ether, 204 SD rats were perfused with 2-4 ℃ Ringer’s solution through the abdominal aorta. In order to reduce warm ischemia of the liver, the liver was not turned over before perfusion. The suprahepatic inferior vena cava was cut off along the phrenic ring after perfusion. No further trimming was needed when dressing, so as not to damage the vena cava. The donor liver was removed and preserved in 4℃Ringer’s liquid. The receptor liver was cut off and allogeneic orthotopic liver transplantation was performed using modified two-cuff method. After transplantation, rats could automatically turn over and drink water. Surviving more than 3 days is regarded as a successful transplantation. RESULTS AND CONCLUSION:102 liver transplantations were performed in 204 rats, with 86 rats surviving more than 3 days. The success rate of transplantation was 84%. The results demonstrate that rat models of orthotropic liver transplantation can be constructed successfully through improving techniques.