银杏达莫注射液联合骨髓间充质干细胞移植改善脑梗死后的神经功能

背景：通过细胞移植重建损伤脑组织成为治疗脑梗死的新途径，骨髓间充质干细胞成为近年来细胞移植治疗领域的研究热点。目的：探讨银杏达莫注射液联合骨髓间充质干细胞移植对脑梗死大鼠神经功能的改善作用及相关机制。方法：利用线栓法制作大鼠大脑中动脉闭塞模型，建模成功后60只SD大鼠随机分为对照组、细胞移植组及联合组。对照组尾静脉注射PBS、细胞移植组尾静脉注射2.5×109 L-1的骨髓间充质干细胞悬液、联合组尾静脉注射2.5×109 L-1的骨髓间充质干细胞悬液和银杏达莫2 mL/kg，1次/d，连续注射5 d。于移植后的1，3 d及1，2 周进行mNSS行为学评分，以观察大鼠神经功能缺损状况。移植后2周RT-PCR检测脑组织中脑源性神经生长因子、生长相关蛋白43基因表达变化，TUNEL法检测细胞凋亡情况，免疫组化法检测BrdU阳性细胞数。结果与结论：移植后的1，3 d各组大鼠神经功能缺损评分差异无显著性意义(P > 0.05)，在移植后1，2周，联合组神经功能缺损评分低于细胞移植组及对照组(P < 0.05)；移植后2周，联合组脑源性神经生长因子、生长相关蛋白43 mRNA表达明显高于细胞移植组及对照组(P < 0.05)，联合组凋亡细胞数目明显少于细胞移植组及对照组(P < 0.05)，联合组BrdU阳性细胞数量明显多于细胞移植组及对照组(P < 0.05)。结果表明骨髓间充质干细胞联合银杏达莫干预能促进脑梗死组织脑源性神经生长因子、生长相关蛋白43 mRNA的表达，抑制细胞凋亡，改善大鼠神经功能。 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程 

Ginkgo-damole injection combined with bone marrow mesenchymal stem cell transplantation improves neurologic recovery from cerebral infarction

BACKGROUND: Reconstruction of damaged brain tissue through cell transplantation has become a new way to treat cerebral infarction. In recent years, bone marrow mesenchymal stem cells have become the new darling in cell transplantation therapy.OBJECTIVE: To investigate the effect of ginkgo-damole injection combined with bone marrow mesenchymal stem cell transplantation to improve the neurological function of acute cerebral infarction rats and its mechanism.METHODS: Animal models of middle cerebral artery occlusion were made in rats using suture method, and then 60 rat models were randomly divided into control group, cell transplantation group and combination group. The control group was given intravenous injection of PBS via the tail vein; the cell transplantation group was given intravenous injection of bone marrow mesenchymal stem cell suspension (2.5×109/L) via the tail vein; the combination group was given intravenous injection of bone marrow mesenchymal stem cell suspension (2.5×109/L) and ginkgo-damole injection (2 mL/kg, once a day, totally 5 days) via the tail vein. Modified  neurological severity scores were recorded at 1, 3 days and 1, 2 weeks after transplantation. At 2 weeks after transplantation, expressions of brain-derived neurotrophic factor and growth associated protein 43 in the brain were detected using RT-PCR; cell apoptosis detected using MTT assay; BrdU positive cells counted using immunohistochemistry method.RESULTS AND CONCLUSION: There were no differences in the modified neurologic severity scores among the three groups at 1, 3 days after transplantation (P > 0.05), but the modified neurological severity scores in the combination group were lower than those in the cell transplantation group and control group at 1, 2 weeks after transplantation (P < 0.05). The expressions of brain-derived neurotrophic factor and growth associated protein 43 in the brain were significantly higher in the combination group than the other two groups at 2 weeks after transplantation (P < 0.05); compared with the other two groups, the number of apoptotic cells was less but the number of BrdU positive cells was higher in the combination group (P < 0.05). These findings indicate that the combination of ginkgo-damole injection and bone marrow mesenchymal stem cell transplantation can increase the expressions of brain-derived neurotrophic factor and growth associated protein 43 in the brain, inhibit cell apoptosis and improve neurological function in rats with cerebral infarction.