调节骨髓间充质干细胞向皮肤创面迁移过程中的血小板衍生生长因子

背景：血小板衍生生长因子具有创伤修复作用，对其研究大部分集中于骨组织的修复，在皮肤创伤愈合中的修复作用研究较少。 目的：观察血小板衍生生长因子在皮肤创伤愈合过程中调控骨髓间充质干细胞向创面迁移促进创面愈合的作用。 方法：培养大鼠骨髓间充质干细胞，免疫荧光检测细胞表面标记物CD34、CD44。选取健康雄性SD大鼠30只，随机分为5组，各组大鼠尾静脉注射PKH26标记的骨髓间充质干细胞。注射1周后，于大鼠背部正中线划长为3 cm的切口，制备皮肤创伤模型。造模后即刻于皮肤创伤处多点注射不同质量浓度血小板衍生生长因子干预药物，对照组注射等体积的生理盐水。注射14 d后留取皮肤创面组织进行相关指标检测。 结果与结论：荧光显微镜下观察血小板衍生生长因子能够剂量依赖性诱导骨髓间充质干细胞向皮肤创伤组织处迁移和聚集，进而促进皮肤创伤修复。Masson染色结果显示，随着血小板衍生生长因子干预质量浓度的增加，创面炎性细胞浸润减轻、胶原纤维数量不断增多。Western blot检测结果显示，血小板衍生生长因子能够抑制皮肤创伤组织基质金属蛋白酶1的表达，促进基质金属蛋白酶组织抑制因子1的表达，抑制胶原降解，发挥间接促愈合作用。中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程全文链接：

Platelet-derived growth factor promotes skin wound healing by regulating the migration of bone marrow mesenchymal stem cells to wounds

BACKGROUND:Platelet-derived growth factor has the ability of wound repair, and relevant studies mainly focus on bone tissue repair. However, there are less studies about the effect of platelet-derived growth factors in skin wound healing. OBJECTIVE:To investigate the role of platelet-derived growth factor to promote wound healing by the regulation of bone marrow mesenchymal stem cells to the wound. METHODS:Bone marrow mesenchymal stem cells from rats were cultured. Immunofluorescence method was conducted to detect cell surface markers of CD34 and CD44 in bone marrow mesenchymal stem cells. Thirty healthy male SD rats were divided into five groups at random. Bone marrow mesenchymal stem cells labeled with PKH-26 were injected into the rat caudal vein in each group. The rats were anesthetized 1 week after injection. On the center of rat back, a 3-cm incision was made to establish a wound healing model. Different concentrations of platelet-derived growth factor were injected via multi-points on the skin wound after modeling, and the control group was treated with the same volume of normal saline. Skin wound tissues were taken for relevant parameter measurement at 14 days after injection. RESULTS AND CONCLUSION:Under the fluorescence microscope, platelet-derived growth factor could induce the migration and accumulation of bone marrow mesenchymal stem cells to the trauma in a dose-dependent manner and promote the wound healing. Masson staining showed that, with the concentration increase, platelet-derived growth factors could reduce inflammatory cell infiltration and increase the number of collagen fibers. Results from western blot assay showed that platelet-derived growth factor could inhibit the expression of matrix metalloproteinase-1, promote the expression of tissue inhibitor of matrix metalloproteinase 1 in the wound, and inhibit the collagen degradation, thereby promoting skin wound healing indirectly.