脑缺血再灌注模型大鼠接受跑台运动锻炼海马反义导向分子A的表达

背景：脑卒中后，运动锻炼对其神经功能的康复具有极其重要的作用，但关于卒中后大鼠运动量的研究鲜有报道。而海马与认知功能密切相关，但大鼠缺血再灌注后海马内反义导向分子A的表达的研究，国内外未见报道。 目的：探索运动锻炼对脑缺血再灌注后大鼠缺血侧海马内反义导向分子A表达的影响。 方法：将120只SD大鼠随机等分为正常组、假手术组、模型7，14，28 d组，模型7，14，28 d组大鼠采用线栓法制作大鼠右侧脑缺血再灌注模型。各组大鼠各等分为`4个亚组：非运动亚组、低、中、高运动量亚组。低运动量组进行5 m/min，5 min；7 m/min，5 min；9 m/min，20 min跑台训练；中运动量组进行8 m/min，5 min；10 m/min，5 min；13 m/min，20 min跑台训练；高运动量组进行8 m/min，5 min；11 m/min，5 min；20 m/min，20 min跑台训练。 结果与结论：非运动时，模型7 d组大鼠缺血侧海马反义导向分子A mRNA和蛋白表达水平最高，且反义导向分子A相对表达水平随时间延长而逐渐降低。与非运动相比，模型14，28 d组大鼠中运动量时缺血侧海马反义导向分子A mRNA和蛋白表达水平明显下降(P < 0.05)，而高运动量海马反义导向分子A mRNA和蛋白表达水平表达增加。提示中运动量锻炼可以降低脑缺血再灌注大鼠患侧海马反义导向分子A的表达。 中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程全文链接：

Expression of repulsive guidance molecule A in the hippocampus of rat models of cerebral ischemia-reperfusion injury during treadmill exercise

BACKGROUND:Exercises play an important role in the recovery of neurological function after stroke. Few studies concerned the amount of exercise in rats after stroke. Hippocampus is strongly associated with cognitive function, but no reports addressed the expression of repulsive guidance molecule A in the rat hippocampus after ischemia and reperfusion. OBJECTIVE:To explore the effects of exercise on repulsive guidance molecule A expression in the hippocampus on the ischemic side in rats after cerebral ischemia-reperfusion injury. METHODS:120 Sprague-Dawley rats were equally and randomly divided into normal group, sham-operation group, and 7-, 14-, 28-day model groups. The model of right cerebral ischemia-reperfusion injury was induced by ligation with nylon monofilament in rats of 7-, 14-, 28-day model groups. Low exercise group received treadmill training of 5 m/min, 5 minutes; 7 m/min, 5 minutes; 9 m/min, 20 minutes. Moderate exercise group received treadmill training of 8 m/min, 5 minutes; 10 m/min, 5 minutes; 13 m/min, 20 minutes. High exercise group received treadmill training of 8 m/min, 5 minutes; 11 m/min, 5 minutes; 20 m/min, 20 minutes.  RESULTS AND CONCLUSION:Repulsive guidance molecule A mRNA and protein expression was highest in the ischemic side of the hippocampus in the 7-day model group without excercise. Moreover, repulsive guidance molecule A relative expression gradually reduced over time. Compared with non-exercise, repulsive guidance molecule A mRNA and protein expression significantly decreased in the 14- and 28-day model groups during moderate exercise (P < 0.05), but repulsive guidance molecule A mRNA and protein expression increased during high exercise. Above data confirmed that moderate exercises could decrease repulsive guidance molecule A  expression in the affected side of the hippocampus of rats with cerebral ischemia-reperfusion injury.