血管内皮生长因子165转染可促进脂肪间充质干细胞增殖

背景：血管内皮生长因子是目前最重要的促血管新生因子之一，在脂肪移植中可促进移植物的血运重建，提高成活率。 目的：探讨血管内皮生长因子165基因转染促进脂肪间充质干细胞增殖的作用。 方法：利用目的片段重组血管内皮生长因子165基因入腺病毒pAdEasy-1系统，包装病毒并测定滴度，同理包装空病毒。将包装成功的两种病毒液分别以100的最佳感染复数转染入脂肪间充质干细胞内，以未转染细胞为空白组。采用RT-PCR和Western blot法检测各组转染细胞中血管内皮生长因子165 mRNA和蛋白的表达，MTT法检测各组细胞的增殖情况。 结果与结论：实验组血管内皮生长因子165 mRNA和蛋白表达量高于对照组和空白组(P < 0.05)；实验组转染脂肪间充质干细胞后分裂增殖程度显著增加，与对照组和空白组比较差异有显著性意义(P < 0.05)，结果表明腺病毒介导的血管内皮生长因子165基因转染脂肪间充质干细胞后可以持续表达目的蛋白，同时也能促进脂肪间充质干细胞的显著增殖。 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Vascular endothelial growth factor 165 transfection promotes proliferation of adipose-derived mesenchymal stem cells

BACKGROUND: Vascular endothelial growth factor is the most important pro-angiogenic factor, which can promote revascularization and survival of fat grafts during fat transplantation. OBJECTIVE:To investigate the effect of vascular endothelial growth factor 165 transfection on the proliferation of adipose-derived mesenchymal stem cells. METHODS:Recombinant vascular endothelial growth factor 165 mRNA fragment was transmitted into adenovirus pAdEasy-1 systems that were packaged to measure viral titer (experimental group). Empty adenovirus was also packaged as control group. Two kinds of packaged adenovirus solution were transferred into adipose-derived mesenchymal stem cells at the best multiplicity of infection=100. Cells with no transfection served as blank group. RT-PCR and western blot methods were used to detect the expression of vascular endothelial growth factor 165 at mRNA and protein levels; MTT method was adopted to detect cell proliferation. RESULTS AND CONCLUSION:The expressions of vascular endothelial growth factor 165 mRNA and protein were significantly higher in the experimental group than the control and blank groups (P < 0.05). The division and proliferation of transfected adipose-derived mesenchymal stem cells were increased significantly in the experimental group, which was significantly different from the control and blank groups (P < 0.05). These finding indicate that vascular endothelial growth factor 165 transfection cannot only sustain the target protein expression of adipose- derived mesenchymal stem cells, but also promote the proliferation of adipose-derived mesenchymal stem cells remarkably.