CIAPIN1 gene silencing enhances chemosensitivity in a drug-resistant
animal model in vivo
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| Authors: | XM Wang SJ Gao XF Guo WJ Sun ZQ Yan WX Wang YQ Xu D Lu |
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| Institution: | 1.Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China;2.Department of Breast Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin, China |
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| Abstract: | Overexpression of cytokine-induced apoptosis inhibitor 1 (CIAPIN1) contributes to
multidrug resistance (MDR) in breast cancer. This study aimed to evaluate the
potential of CIAPIN1 gene silencing by RNA interference (RNAi) as a
treatment for drug-resistant breast cancer and to investigate the effect of
CIAPIN1 on the drug resistance of breast cancer in
vivo. We used lentivirus-vector-based RNAi to knock down
CIAPIN1 in nude mice bearing MDR breast cancer tumors and found
that lentivirus-vector-mediated silencing of CIAPIN1 could
efficiently and significantly inhibit tumor growth when combined with chemotherapy
in vivo. Furthermore, Western blot analysis showed that both
CIAPIN1 and P-glycoprotein expression were efficiently downregulated, and P53 was
upregulated, after RNAi. Therefore, we concluded that lentivirus-vector-mediated RNAi
targeting of CIAPIN1 is a potential approach to reverse MDR of
breast cancer. In addition, CIAPIN1 may participate in MDR of breast
cancer by regulating P-glycoprotein and P53 expression. |
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| Keywords: | CIAPIN1 gene Multidrug resistance RNA interference MDR1 gene Breast neoplasms |
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