miR-486对胶质瘤干细胞的抑制作用

背景：既往研究发现，miR-486在胶质瘤干细胞(CD133⁺)中的表达水平显著低于其在胶质瘤非干细胞(CD133^-)中的表达水平，但是miR-486对CD133⁺细胞的影响尚不明确。 目的：探索miR-486对CD133⁺细胞的作用。 方法：利用流式细胞分选将U87胶质瘤细胞中分为CD133⁺和CD133^-细胞。通过脂质体转染构建miR-486过表达的胶质瘤干细胞。 结果与结论：流式细胞分选和纯化获得高比率的CD133⁺胶质瘤干细胞。实时反转录PCR检测发现miR-486在CD133⁺胶质瘤干细胞的表达水平比CD133^-胶质瘤细胞明显下降。脂质体转染成功构建miR-486过表达的胶质瘤干细胞，体外实验发现miR-486高表达抑制胶质瘤干细胞的增殖，将其阻滞于G₁/S期，并促进凋亡。提示miR-486对胶质瘤干细胞具有抑制作用。中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

miR-486 is a tumor suppressor in glioma stem cells

BACKGROUND:Previous studies have found that the expression level of miR-486 in glioma stem cells (CD133⁺) is significantly down-regulated compared with that in glioma non-stem cells (CD133^-), but the effect of down-regulation of miR-486 on CD133⁺ cells remains unclear . OBJECTIVE:To explore the effect of miR-486 on CD133⁺ cells. METHODS:CD133⁺ glioma stem cells and CD133^- glioma cells were separated from U87 cells by flow cytometer. miR-486 overexpression glioma stem cells were constructed by lipofection transfection. RESULTS AND CONCLUSION:After sorting and purification, the content of the CD133⁺ fraction was enriched up to 83.5%. The expression level of miR-468 in CD133⁺ glioma stem cells was obviously down-regulated compared with that in CD133^- glioma cells. CD133⁺ glioma stem cells overexpressing miR-486 were fabricated successfully. Results from in vitro experiments showed that miR-486 overexpression could dramatically decrease the proliferation of glioma stem cells, induce a cell cycle arrest in G1/S phase for CD133⁺ glioma stem cells and promote cell apoptosis. These findings suggest that miR-486 can be a suppressor of glioma stem cells, which offers a novel potential therapeutic target for glioma stem cells and human glioma.