Successful pregnancy outcome after in vitro fertilisation following Pre-implantation Genetic Diagnosis/Polymerase Chain Reaction screening for single gene disorder (sickle cell anaemia) before embryo transfer: The clinical experience of an in vitro fertilisation clinic in Nigeria |
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Authors: | Chizara Okeke Kemi Ailoje-Ibru Kemi Olukoya Rose Ogbeche Abiola Adewusi Ebele Iloabachie Oladapo Ashiru |
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Institution: | 1.Medical Assisted Reproductive Technology Centre, Ikeja, Lagos, Nigeria;2.Medical Assisted Reproductive Technology Centre, University of Illinois, Chicago, Illinois, USA |
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Abstract: | A couple, both carriers of the sickle cell anaemia trait (Genotype HbAS) with an offspring already affected with the genetic disease underwent a Pre-implantation Genetic Diagnosis/Polymerase Chain Reaction screening of biopsied blastomeres. DNA analysis of single blastomeres was carried out to find out indicated a viable intra-uterine pregnancy with embryos which carried the sickle cell mutation, which resulted in a livebirth (HbAS). PGD/PCR in combination with IVF appears to be the most suitable treatment plan for patients who are at a higher risk of reproducing offspring affected with inheritable genetic diseases. |
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Keywords: | PGD/PCR Sickle cell anaemia IVF Biopsy FET |
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