芍药苷对骨髓间充质干细胞增殖的影响

背景：研究显示芍药苷具有补血及治疗自身免疫性疾病的功效，骨髓间充质干细胞对机体的造血及免疫功能也起着重要的作用，但芍药苷对骨髓间充质干细胞的增殖及细胞因子的分泌和表达有何影响报道较少。 目的：探讨芍药苷对人骨髓间充质干细胞增殖及白细胞介素6表达的影响。 方法：采用密度梯度离心法和贴壁培养法体外分离培养人骨髓间充质干细胞，用流式细胞术和成脂及成骨诱导法鉴定人骨髓间充质干细胞生物学特性，MTT法检测不同浓度芍药苷对人骨髓间充质干细胞增殖的影响，ELISA 法测定芍药苷干预人骨髓间充质干细胞后培养上清液中白细胞介素6的分泌水平，RT-PCR 检测芍药苷干预后白细胞介素6 mRNA的表达情况。 结果与结论：成功分离出骨髓间充质干细胞，具有成骨、成脂分化潜能。与对照组相比，芍药苷浓度为2 μmol/L和10 μmol/L可明显促进骨髓间充质干细胞增殖。10 μmol/L芍药苷干预骨髓间充质干细胞后，G₀/G₁期细胞比例显著降低，S期细胞比例显著升高。10 μmol/L芍药苷干预组骨髓间充质干细胞白细胞介素6的分泌和mRNA表达均显著高于对照组(P < 0.01)。由此得出，一定浓度的芍药苷可促进骨髓间充质干细胞增殖，并提高骨髓间充质干细胞分泌白细胞介素6水平和基因表达。中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程全文链接：

Effects of paeoniflorin on the proliferation of bone marrow mesenchymal stem cells

BACKGROUND: Studies have shown that paeoniflorin functions as replenishing blood and treatment of autoimmune diseases, and bone marrow mesenchymal stem cells also play an important role in the body’s blood and immune function. However, paeoniflorin effects on bone marrow mesenchymal stem cell proliferation and cytokine secretion and expression are rarely reported. OBJECTIVE:To investigate the effect of paeoniflorin on proliferation of bone marrow mesenchymal stem cells and the expression of interleukin-6. METHODS:Human bone marrow mesenchymal stem cells were separated and cultured in vitro by density gradient centrifugation combined with attachment method. The biological characteristics of bone marrow mesenchymal stem cells were identified by flow cytometry and osteogenic/adipogenic induction. The proliferation of bone marrow mesenchymal stem cells under different concentrations of paeoniflorin was detected by MTT method. The mRNA expression and secretion of interleukin-6 in the supernatant of bone marrow mesenchymal stem cells were detected by RT-PCR and ELISA, respectively. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cells were isolated successfully and had osteogenic and adipogenic differentiation potential. Compared with the control group, 2 μmol/L and 10 μmol/L paeoniflorin significantly promoted the proliferation of bone marrow mesenchymal stem cells. 10 μmol/L paeoniflorin could significantly decrease the proportion of bone marrow mesenchymal stem cells in G0/G1 phase and increase this proportion in S phase. Compared with the control group, the experimental group could significantly increase the secretion and mRNA expression of interleukin-6 (P < 0.01). It is concluded that paeoniflorin at certain concentrations can obviously promote the proliferation of bone marrow mesenchymal stem cells, and increase the expression and secretion of interleukin-6.