| 瘢痕组织成纤维细胞培养及其超微结构观察 |
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| 引用本文： | 黄勇,林立新,赵伟,陈才远,周爱东. 瘢痕组织成纤维细胞培养及其超微结构观察[J]. 中国组织工程研究与临床康复, 2001, 5(14): 54 |
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| 作者姓名： | 黄勇 林立新 赵伟 陈才远 周爱东 |
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| 作者单位： | 烟台毓璜顶医院,山东烟台 264000 |
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| 摘 要： | 目的观察不同瘢痕组织体外成纤维细胞培养其形态结构的异同.方法切取正常皮肤、增殖性瘢痕和瘢痕疙瘩组织行体外成纤维细胞培养,观察其形态和超微结构.结果三类成纤维细胞大体形态学方面没有明显差异,但增殖性瘢痕和瘢痕疙瘩成纤维细胞胞质中含有发达的粗面内质网和高尔基复合体,其增殖性瘢痕成纤维细胞还含有大量肌微丝,具有肌成纤维细胞(Myofibroblast,MF)特征.结论各类瘢痕成纤维细胞在体外培养状况下,其形态学表现稍有不同.
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| 关 键 词： | 增 殖 性 瘢 痕 瘢 痕 疙 瘩 细 胞 培 养 肌 成 纤 维 细 胞 |
| 文章编号： | 1007-5496(2001)07-0054-01 |
| 修稿时间： | 2001-02-12 |
Observation of the ultrostructures of cicatricial fibroblasts in culture |
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| Abstract: | Objective The experiment was designed to observe the morphologies of fibroblasts isolated from different scars. Method The fibroblasts from skin, hypertrophic scar and keloid were cultured, respectively, in vitro, and their morphologies and ultrostructures were compared. Result The results showed that there was no significant difference of the cellular morphologies of fibroblasts in the 3 groups.The fibroblasts of hypertrophic scar and keloid contained abundant rough endoplasmic reticulum and Golji complexes. Many fine myofilanents were present in hypertrophic scar fibroblast having the characteristics of myofibroblast. Conclusion There was no significant difference of the cellular morphologies in different cicatricial fibroblasts in culture. |
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| Keywords: | hypertrophic scar keloid cell culture myofibroblast |
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