| 氮源对庆大霉素合成与分泌的影响 |
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| 引用本文： | 张达力,储炬,李友荣. 氮源对庆大霉素合成与分泌的影响[J]. 医学教育探索, 2002, 0(4): 360-363 |
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| 作者姓名： | 张达力 储炬 李友荣 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室 上海200237(张达力，储炬)，华东理工大学生物反应器工程国家重点实验室 上海200237(李友荣) |
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| 基金项目： | 国家自然科学基金资助项目 ( 39870 0 18 |
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| 摘 要： | 研究了无机与有机氮源对庆大霉素合成与分泌的影响。分别在初始培养基中及进入生物合成期的发酵液中添加硫酸铵、硝酸钠和各种氨基酸，初始培养基中添加硫酸铵浓度为0.1g/L时，总效价提高了23％，同时，硫酸铵对庆大霉素组分也有一定影响。在培养至24h添加8g/L硝酸钠，总效价提高55％，而在初始培养基中分泌率提高了33％，在生物合成期添加0.8g/L赖氨酸，总效价提高了64％，同时在静息细胞培养基中进行验证实验。
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| 关 键 词： | 氮源 庆大霉素 合成 分泌 广谱抗生素 初始培养基 硫酸铵 硝酸钠 氨基酸 |
Influence of Nitrogen Sources on Synthesis and Secretion of Gentamicin |
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| ZHANG Da li,CHU Ju,LI You rong. Influence of Nitrogen Sources on Synthesis and Secretion of Gentamicin[J]. Researches in Medical Education, 2002, 0(4): 360-363 |
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| Authors: | ZHANG Da li CHU Ju LI You rong |
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| Abstract: | The effects of inorganic and organic nitrogen sources on synthesis and secretion of gentamicin in shake flask fermetation were studied. (NH 4) 2SO 4,NaNO 3 and various amino acids were added initially and in biosynthesis phase. The results showed that total potency increased by 23% when the concentration of (NH 4) 2SO 4 was 0.1g/L; component of gentamicin was also influenced by (NH 4) 2SO 4; total potency incresased by 55% when 8g/L NaNO 3 was added at 24h; secretion ratio increased by 33% when 8g/L NaNO 3 was added intially; total potency increased by 64% when 0.8g/L lysine was added in the biosynthesis phase. At the same time, verified experiment was done in the resting cell system. |
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| Keywords: | nitrogen source gentamicin synthesis secretion |
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