倍增差示分光光度法测定复方甲硝唑溶液含量

选择λ278nm 和λ300nm 作为甲硝唑和氯霉素二药的定量波长,通过两波长下吸光系数和配平倍数的测算,由工作曲线方程求得样品含量。实验测得甲硝唑和氯霉素的回收率分别为100.13%和99.73%,变异系数分别为0.3%和0.17%,说明方法可行。

Determination of the Content of Compound Metronidazole Solution by Double Differential Spectrophotometry

In accordance with the principle of two wavelength coefficient double spectroph- otometry,the Double Differential Spectrophotometry was used for the determination of the component,metronidazole and Chloramphenicolis,that is interferential each other.The det- erminating wavelength was choosed at 278nm and 300nm.The sample's content was calculated by the working curve equation on the basis of the determination of absobent coeffi(?)ient and match multiple at this two wavelength.The method was easier,simpler,faster and accurate. The average recovery rate of metronidazole and Chloramphenicolis was separately 100.13% and 99.73%,CV 0.3% and 0.17%,explaining the method useful.