| 原儿茶醛对ox-LDL损伤的血管内皮细胞保护作用 |
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| 引用本文： | 韩纯洁,林蓉,刘俊田,刘瑜,张慧.原儿茶醛对ox-LDL损伤的血管内皮细胞保护作用[J].中药材,2007,30(12):1541-1544. |
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| 作者姓名： | 韩纯洁 林蓉 刘俊田 刘瑜 张慧 |
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| 作者单位： | 1. 西安交通大学,医学院,陕西西安,710061
2. 西安交通大学,医学院,陕西西安,710061;西安交通大学,环境与疾病相关基因教育部重点实验室,陕西西安,710061 |
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| 基金项目： | 国家自然科学基金(90709018)，西安市科技攻关项目(GG06174) |
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| 摘 要： | 目的:观察原儿茶醛对ox-LDL诱导的人脐静脉血管内皮细胞株(CRL-1730)损伤的保护作用。方法:ox-LDL诱导人血管内皮细胞损伤,并以不同剂量的原儿茶醛干预24 h,用四甲基偶氮唑蓝比色法检测细胞存活数量,比色法测定细胞培养液中NO含量和NOS活性,流式细胞术测定细胞内CD40蛋白表达。结果:原儿茶醛对ox-LDL引起的CRL-1730损伤血管内皮细胞数量的减少和培养液中NO、NOS的降低有明显的抑制作用;ox-LDL可引起细胞内CD40蛋白表达增加,原儿茶醛可以抑制此作用。结论:原儿茶醛对ox-LDL诱导的血管内皮细胞损伤具有保护作用,其机制可能与CD40/CD40L抗炎途径有关。
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| 关 键 词： | 原儿茶醛 血管内皮细胞 |
| 文章编号： | 1001-4454（2007）12-1541-04 |
| 收稿时间： | 2007-04-23 |
| 修稿时间： | 2007年4月23日 |
Protection of Vascular Endothelial Cells from ox-LDL Induced Injury by Protocatechualdehyde |
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| HAN Chun-jie,LIN Rong,LIU Jun-tian,LIU Yu,ZHANG Hui.Protection of Vascular Endothelial Cells from ox-LDL Induced Injury by Protocatechualdehyde[J].Jorunal of Chinese Medicinal Materials,2007,30(12):1541-1544. |
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| Authors: | HAN Chun-jie LIN Rong LIU Jun-tian LIU Yu ZHANG Hui |
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| Institution: | Xián Jiaotong University, China. |
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| Abstract: | Objective:To observe the protective effects of protocatechualdehyde on the human umbilical vein endothelial cells(CRL-1730) induced injury by ox-LDL.Methods:The CRL-1730 were induced injury by ox-LDL,and then treated with protocatechualdehydes for 24 hours.The cytoactive of CRL-1730 was assessed by colorimetry of MTT,the NO level and NOS activity in the cell culture fluid were observed by colorimetry,and the expression of CD40 protein was determined by flow cytometry.Results:Compared with the ox-LDL group,protocatechualdehyde increased the number of CRL-1730 and the level of NO and NOS in cell culture fluid.Besides,protocatechualdehyde decreased the expression of CD40 protein,which was increased by oxLDL.Conclusion:Protocatechualdehyde has protective effect on the CRL-1730 induced injury by ox-LDL and its mechanism of action may be related to the CD40/CD40L pathway. |
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| Keywords: | ox-LDL CD40 |
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