肝星状细胞激活与ICAM-1的表达

目的探讨肝星状细胞（ＨＳＣ）的活化与细胞间粘附分子１（ＩＣＡＭ１）表达的关系．方法用链酶蛋白酶和胶原酶原位灌流，Ｎｙｃｏｄｅｎｚ密度梯度离心分离大鼠ＨＳＣ，并进行体外培养，应用免疫组织化学方法观察静息或活化状态下的ＨＳＣ中ＩＣＡＭ１表达．结果静息的ＨＳＣ不表达ＩＣＡＭ１，而活化的ＨＳＣ表达ＩＣＡＭ１，且随着培养时间的延长ＩＣＡＭ１表达量逐渐增加．结论ＩＣＡＭ１表达与ＨＳＣ活化及肝纤维化的发生有关．

Expression of intercellular adhesion molecule 1 by activated hepatic stellate cells *

AIM To explore the relationship between activation of hepatic stellate cells (HSC) and expression of intercellular adhesion molecule 1(ICAM 1). METHODS HSCs were isolated and cultured from liver of Wistar rats by in situ perfusion with pronase and collagenase and density gradient centrifugation with Nycodenz. Expression of ICAM 1 in a quiescent and an activated HSC was detected by immunohistochemistry assay. RESULTS Expression of ICAM 1 was not observed in the quiescent HSC, but seen in the activated, and the amount of the expression was increasing with the time of culture. CONCLUSION Expression of ICAM 1 is related to activation of HSC and liver fibrosis.