The synthesis of tenuazonic acid o‐carboxymethyl oxime and its conjugation to macromolecules |
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| Authors: | A. E. John R. D. Coker G. Blunden D. J. Rogers T. A. Crabb |
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| Affiliation: | 1. Mycotoxins Section , Natural Resources Institute, Central Avenue , Chatham Maritime, Kent, ME4 4TB, UK;2. Portsmouth Polytechnic , School of Pharmacy and Biomedical Sciences , King Henry 1 Street, Portsmouth, Hants, PO1 2DZ, UK;3. Portsmouth Polytechnic , School of Chemistry , White Swan Road, Portsmouth, Hants, PO1 2DT, UK |
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| Abstract: | The o‐carboxymethyl oxime derivative of the mycotoxin and phytotoxin, tenuazonic acid, has been synthesized and characterized by 1H‐NMR (nuclear magnetic resonance) and IR spectroscopy and mass spectrometry. The derivative contains a carboxylic acid moiety which has been conjugated to bovine serum albumin and a modified bovine serum albumin, using the mixed anhydride method, in toxin‐to‐protein ratios of 15:1 and 30:1 respectively. Isoelectric focusing was used to demonstrate that the conjugation process had been successfully achieved. The two conjugates appear to be suitable for the preparation of either polyclonal or monoclonal antibodies to tenuazonic acid for use in the development of immunoassays for this toxin. |
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| Keywords: | tenuazonic acid tenuazonic acid o‐carboxymethyl oxime conjugates to macromolecules polyclonal antibodies |
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