结晶型硫化镍诱导的人支气管上皮细胞系转化细胞基因组不稳定性分析

目的　检测结晶型硫化镍对基因组不稳定性的影响 ,从而进一步探讨镍化合物致癌的分子机制。方法　采用随机扩增多态性DNA(randomamplifiedpolymorphicDNA ,RAPD)技术对经结晶型硫化镍在诱导人支气管上皮细胞系 (16HBE)的恶变细胞中的基因组不稳定性进行分析。结果　本实验所选用的 7条随机引物均能扩增出清晰、明显的条带 ,条带数在 1～ 6条之间。 7条引物中第 4条、第 5条和第 7条两条扩增的片段在实验组和对照组之间无明显差异 ,其余 4条引物均有差异。对于同一随机引物他们都具有特异的带型。结论　结晶型硫化镍能诱发 16HBE细胞基因组不稳定

Crystalline nickel sulfide-induced genomic instability in transformed human broncho-epithelial cells

OBJECTIVE: To detect the genomic instability in the 16 human broncho-epithelial (16HBE) cells induced by crystalline nickel sulfide so as to provide the scientific basis for further study of nickel-induced cancer molecular mechanism. METHODS: To analyse the genomic instability in transformed 16HBE cells induced by crystalline nickel sulfide by random amplified polymorphic DNA (RAPD). RESULTS: All the 7 random primers selected could amplify 1 - 6 clear PCR bands. There were no significant differences between transformed 16HBE cells and negative control cells in the 4th, 5th, and 7th primers, but in the rest 4 primers there were significant differences, with special PCR bands for the same primer, indicating that genomic instability in transformed 16 HBE cells was induced by crystalline nickel sulfide. CONCLUSION: Crystalline nickel sulfide could induce genomic instability in 16HBE cells.