麻黄-葛根药对治疗糖尿病周围神经病变的药理作用

目的:利用中药整合药理学平台及分子对接研究探讨麻黄-葛根药治疗糖尿病周围神经病变的分子机制.方法:首先在疾病靶点筛选数据库和中药系统药理学数据库与分析平台(TCMSP)检索获取与糖尿病周围神经病变(DPN)相关的靶标基因及麻黄、葛根的活性成分;然后使用Excel查重获得药物与疾病的共同靶点,使用Cytoscape绘制蛋...

Study on the Pharmacological Mechanism of Herba Ephedrae and Radix Puerariae on the Treatment of Diabetic Peripheral Neuropathy

Objective:To explore the molecular mechanism of Herba Ephedrae and Radix Puerariae drug pair in the treatment of diabetic peripheral neuropathy by using the integrated pharmacology platform of traditional Chinese medicine and molecular docking research.Methods:Firstly searched the disease target screening database and TCMSP platform to obtain the related target genes of DPN,Herba Ephedrae and Radix Puerariae and the active ingredients of the latter;then use excel to checked the common targets of drugs and diseases,and used Cytoscape to draw PPI Network diagram,GO enrichment analysis of common targets,and KEGG pathway analysis through DAVID website;finally used Cytoscape software to construct a“drug-active ingredient-key target-Gene”relationship network,and use the Systems Dock website for molecular docking to evaluate the stability of active ingredients and target binding.Results:Herba Ephedrae and Radix Puerariae drug pair had 21 active ingredients that act on diabetic peripheral neuropathy,and 138 common target genes.The results of the drug′s treatment of DPN enrichment mainly focused on removal of Among the seven functional groups such as removal of superoxide radicals,the main enriched pathways included PKC signaling pathway,Polyol pathway activation,etc.The main therapeutic target is PKC,PTGS2,Nrf2,etc.Conclusion:Herba Ephedrae and Radix Puerariae may be treated by anti-oxidative stress and inflammatory response,slowing down the damage of nerve myelin and Schwann cells,and improving microcirculation.