神经细胞老化过程中蛋白质组学研究

目的 研究神经细胞老化过程中细胞内蛋白质表达的变化.方法 采用无血清培养神经母细胞瘤细胞的方法建立实验性神经细胞老化模型,设立12 d(老化)组和5 d(对照)组;运用蛋白质组学技术平台,采用双向电泳结合基质辅助激光电离-飞行时间质谱技术,分析并鉴定神经细胞老化过程中表达变化的蛋白.结果 与5 d组比较,在12 d组中表达下降的蛋白质点21个,表达升高的蛋白质点19个.成功鉴定出其中12个蛋白质点,其中VIME、DLDH、FSCN1和GSTA4在12 d组中表达上升.TPM4、STMN1、TERA、PSA5、HSP90B、SERB、SYG和PARK7在12 d组中表达下降.结论 神经细胞老化机制涉及对STMN1、PARK7和DLDH等相关蛋白的调控.

Proteomics analysis of neuron during aging

Objective To investigate the changes of protein expression during neuronal aging. Methods The experimental models of neuronal aging were established using serum-free culture of mouse neuroblastoma cells and were divided into 12 d group (aging group) and 5 d group (control group). The protein levels which changed during aging were analysed and identified using two-dimensional electrophoresis with matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Results Compared with 5 d group, the expression of 19 proteins increased, and that of 21 proteins decreased in 12 d group. Twelve proteins were successfully identified. The expression of VIME, DLDH, FSCN1 and GSTA4 increased, and that of TPM4, STMN1, TERA, PSA5, HSP90B, SERB, SYG and PARK7 decreased in 12 d group. Conclusion The molecular mechanisms of neuronal aging are involved in regulation of proteins such as STMN1, PARK7 and DLDH.