| 胶体金免疫渗滤法检测日本血吸虫病血清抗体的研究 |
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| 引用本文： | 许静,严自助,张瑞鉴,冯婷,王强,钱翠珍,吴晓华,朱丹,郭家钢,周晓农. 胶体金免疫渗滤法检测日本血吸虫病血清抗体的研究[J]. 中国寄生虫学与寄生虫病杂志, 2006, 24(2): 146-147,155 |
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| 作者姓名： | 许静 严自助 张瑞鉴 冯婷 王强 钱翠珍 吴晓华 朱丹 郭家钢 周晓农 |
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| 作者单位： | 1. 中国疾病预防控制中心寄生虫病预防控制所,上海,200025
2. 福建省三明博峰生物科技有限公司,三明,365000 |
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| 摘 要： | 应用建立的胶体金免疫渗滤法检测日本血吸虫病血清抗体，并以快速酶联免疫吸附试验（F-ELISA）方法检测作平行对照。现场和实验室试验表明，胶体金渗滤法与F?鄄ELISA方法在敏感度和特异度的差异无统计学意义（P>0.05），两法具有较高的一致性。但胶体金免疫渗滤法操作简便快速，无特殊要求，在临床和现场防治工作中具有比较广泛的应用前景。
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| 关 键 词： | 胶体金免疫渗滤法 快速酶联免疫吸附试验 日本血吸虫病 抗体 |
| 文章编号： | 1000-7423（2006）-02-0146-03 |
| 收稿时间： | 2005-09-16 |
| 修稿时间： | 2005-09-16 |
Antibody Detection in Sera of Patients with SchistosomiasisJaponica by Dot Immunogold Filtration Assay |
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| XU Jing,YAN Zi-zhu,ZHANG Rui-jian,FENG Ting,WANG Qiang,QIAN Cui-zhen,WU Xiao-hua,ZHU Dan,GUO Jia-gang,ZHOU Xiao-nong. Antibody Detection in Sera of Patients with SchistosomiasisJaponica by Dot Immunogold Filtration Assay[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2006, 24(2): 146-147,155 |
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| Authors: | XU Jing YAN Zi-zhu ZHANG Rui-jian FENG Ting WANG Qiang QIAN Cui-zhen WU Xiao-hua ZHU Dan GUO Jia-gang ZHOU Xiao-nong |
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| Affiliation: | 1 National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China; 2 Bofeng Biological and Technical Limited Company, Sanming City, Fujian Province, Sanming 365000, China |
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| Abstract: | Serum antibody of schistosomiasis patients was detected by dot immunogold filtration method(DIGFA)in laboratory and field,and F-ELISA was used as control.The results showed that there was no significant difference between these two assays in sensitivity and specificity(P>0.05),with a high coincidence.DIGFA is easy to operate and may deserve a wide application in the diagnosis of schistosomiasis. |
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| Keywords: | Dot immunogold filtration assay F-ELISA Schistosomiasis japonica Antibody |
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