| 应用PCR—SSP技术对粒细胞抗原NA进行基因分型 |
| |
| 引用本文： | 金蕾 傅启华. 应用PCR—SSP技术对粒细胞抗原NA进行基因分型[J]. 上海医学检验杂志, 1999, 14(6): 334-335 |
| |
| 作者姓名： | 金蕾 傅启华 |
| |
| 作者单位： | 浙江省血液中心!310020 |
| |
| 摘 要： | 根据ＮＡ基因序列，合成ＮＡ特异性引物，通过ＰＣＲ－ＳＳＰ技术，特异性地扩增ＮＡ１和ＮＡ２，建立ＮＡ基因分型方法，同时对１２０例浙江汉族人群进行ＮＡ基因型的检测。结果表明ＰＣＲ－ＳＳＰ技术对ＮＡ的基因分型方法简单、翰林折汉族人群的ＮＡ基因型ＮＡ１为０．５２５，ＮＡ２为０．４７５。
|
| 关 键 词： | 聚合酶链反应 中性粒细胞抗原 基因分型 |
Typing of the granulocyte-specific NA antigens by polymerase chain reaction with sequence-specific primers. |
| |
| Jin Lei,Fu Qihua,Yan Lixing. Blood Centre of Zhejiang Province.. Typing of the granulocyte-specific NA antigens by polymerase chain reaction with sequence-specific primers.[J]. Shanghai Journal of Medical Laboratory Sciences, 1999, 14(6): 334-335 |
| |
| Authors: | Jin Lei Fu Qihua Yan Lixing. Blood Centre of Zhejiang Province. |
| |
| Affiliation: | Jin Lei,Fu Qihua,Yan Lixing. Blood Centre of Zhejiang Province. 310020 |
| |
| Abstract: | According to the NA gene sequences, the NA sequence specific primers were synthesized. Using the polymerase chain reaction with sequence specific primers(PCR SSP) technique, a method for determination of NA genotype was established. The genomic DNA of 120 Zhejiang Han unrelated individuals were typed for NA. The results showed this method was simple, rapid and reliable. The gene frequencies were 0 525 for NA1 and 0 475 for NA2 in Zhejiang Han population. |
| |
| Keywords: | Polymerase chain reaction Neutrophil antigens Genotyping |
| 本文献已被 CNKI 维普 等数据库收录！ |
