糖尿病患者血清低密度脂蛋白亚组分的分布及氧化易感性

为探讨糖尿病（ＤＭ）患者血浆低密度脂蛋白（ＬＤＬ）亚组分的异常特性。本文采用密度工超速离心直接分离ＬＤＬ亚组分，并对其分布及体外铜离子介导的氧化易感性进行分析。结果表示单纯ＤＭ患者、ＤＭ合并症患者及对照组间ＬＤＬ亚组分分布无差别；ＤＭ患者ＬＤＬ各组分氧化延滞时间显著缩短，尤以ＤＭ合并症患者明显，示其易于氧化，血浆脂质巡氧化程度亦增高。认为ＤＭ患者动脉粥样硬化的高发生率同其ＬＤＬ易于氧化的我相关联。

Distribution of low density lipoprotein subfractions and their susceptibility to in vitro oxidation in patients with diabetes mellitus.

To study the distribution of low density lipoprotein (LDL) subfractions and their susceptibility to in vitro oxidation in patients with diabetes mellitus (DM), LDL subtractions were isolated directly from plasma by nonequilibrium density gradient centrifugation, and distributions of LDL subfractions were analyzed. The continuous monitoring of LDL oxidation by absorbance measurements at 234 nm was used to analyze the susceptibility of LDL subfractions to Cu 2 mediated oxidation in patients with DM. It is showed that the frequency distributions of LDL subfractions had no different among simple DM, complicated DM patients and controls. The lag time in the test for LDL oxidizability in DM patients was significantly shorter than that in control. And the lag time in DM complication patients was the shortest. Circulating LDL isolated from DM patients was more susceptible to oxidation in vitro than that of controls. This may be associated with the high level of oxidative modificdtion LDL, and may also be associated with increased risk for coronary artery disease.