3种烤瓷冠基底合金对HGF体外培养模型中IL-1β释出的影响

目的：通过测定人牙龈成纤维细胞（HGF）在体外培养过程中炎症因子白细胞介素-1β（IL-1β）释出量,对不同烤瓷冠基底金属合金进行对比,判断其对牙龈的作用。方法：以镍铬合金、含钛镍铬合金和纯钛为实验材料,在人牙龈成纤维细胞体外培养系统中引入不同金属浸提液,通过酶联免疫吸附试验（ELISA）测定细胞暴露于浸提液1、3、7、24h细胞分泌IL-1β的量。结果：镍铬合金和含钛镍铬合金浸提液使HGF分泌IL-1β量明显增加（P〈0.01）,纯钛组浸提液不影响HGF分泌IL-1β的量,与空白对照组无显著差异。结论：镍铬合金和含钛镍铬合金可促进HGF分泌IL-1β,纯钛材料不影响HGF对IL-1β的分泌、提示其良好的细胞相容性。

Influence of Three Dental Casting Alloys on the Synthesis of IL-1 by HGF in vitro

Objective：To determine the influence of various types of dental casting alloys upon the synthesis of IL-1βin a cell culture system consisting of human gingival fibroblast,and to determine their effect in gingival inflammation.Methods：Ni-Cr,Ni-Cr-Ti and Ti were used as test materials.The materials were exposed to a cell culture in order to determine their effect on IL-1secretion level.Aliquots were taken from exposed media after 1h,3h,7h,24h.Assays for IL-1were carried out by ELISA.Results：Ti had no significant influence on IL-1secretion compared to untreated control cultures.Ni-Cr,and Ni-Cr-Ti increased IL-1levels two folds compared to untreated control cultures.Conclusion：Our findings suggested that Ti did not cause elevated IL-1release from cells.On the other hand,Ni-Cr and Ni-Cr-Ti caused increase in IL-1levels.