血管内皮生长因子在异位和在位子宫内膜中的表达

目的研究血管内皮生长因子（vascular endothelial growth factor,VEGF）在子宫内膜异位症（endometrio-sis,EM）患者的异位和在位内膜中的表达及其在EM发病机制中的作用。方法应用ELISA法检测有或无EM的妇女的血管内皮生长因子（VEGF）水平、采用免疫组织化学S-P法测定VEGF在EM患者异位与在位内膜及对照组增殖期和分泌期子宫内膜组织中的表达,并以抗第八因子相关抗原（F8-RA）抗体标记间质微血管并进行微血管计数（MVD）。结果在整个月经周期中,研究组异位和在位内膜中VEGF表达均持续高于对照组（P〈0.01）。EMs患者异位内膜和在位内膜的微血管密度（MVD）明显高于对照组内膜（P〈0.05）。结论VEGF在EMs患者的高表达,说明其通过促血管生成在该病发生发展中起着重要作用。

Expression of vascular endothelial growth factor in ectopic and eutopic endometrium in patients with endometrios

Objective： To study the expression of vascular endothelial growth factor（VEGF） in ectopic and eutopic endometrium in patients with endometrios,and its role in thepathogenesis of endometriosis.Methods： VEGF concentrations in women with EM and without were measured with ELISA.Immunochemical method was used to determine the expression of VEGF in proliferative phase and secretive phase of ectopic and eutopic endometrium from patientswith EM and the endometrium of controls.Microvessel density（MVD） was evaluated by using anti factorⅧ-related antigen（F8-RA） antibodytomark stromal microvessels.Result： The expression rate of VEGFwas persistently higher in ectopic endometrium and eutopic endometrium than that of control group（P〈0.01）,.The microvessel density（MVD） was markedly higher in the ectopic and eutopic endometrium ofendometriosis than that in the controlgroup（P〈0.05）.Conclusion：The increased VEGF expression in the endometriosis indicates thatVEGF,by promoting the angiogenesis,plays an important role in the occurrence and developmentofendometriosis.