肝癌体外药物敏感性试验

目的 通过测定肝癌对阿霉素（ADM）、顺铂（DDP）、氟尿嘧啶（FU）、长春新碱（VCR）、丝裂霉素C（MMC）及噻替哌（TSPA）的体外药物敏感性，为肝癌化疗用药提供参考依据。方法 采用滤纸支持组织块培养－MTT终点－计算机图像分析体外药物敏感性试验法。结果 在1倍血浆峰浓度（1 PPC）时作用最强的药物为MMC，其抑制率中位数（MIR）为26．5％，其次是ADM为13．0％，DDP、TSPA、5－FU和VCR为4．0－8．5％。在5倍血浆峰浓度（5PPC）时作用最强的药物亦为MMC，其MIR为92．0％，次为ADM、DDP和TSPA为41－50％，5－FU和VCR为16．0－17．0％，DDP和TSPA在5PPC的MIR比在1PPC时大5．0和5．8倍以上，MMC、ADM帮VCR则大2－3．5倍，5－FU只增大1倍左右。结论 结果提示MMC对肝癌抑制作用较强，DDP和TSPA剂量增加与疗效提高可能有较大的关系。天然来源的药物ADM、MMC和VCR之间，无论在1PPC或5PPC水平，其相关系数均较大（r=0.5539-0.7208)，表明部分肝癌具有多药耐药性。

IN VITRO DRUG SENSITIVITY OF HEPATOMA TO SEVERAL CHEMOTHERAPEUTIC AGENTS

Objective　Through assessing the in vitro sensitivity of hepatoma to adriamycin (ADM), cisplatin (DDP), 5-fluorouracil (FU), vinscristine (VCR), mitomycin-C(MMC) and thiotepa (TSPA), reference for chemotherapy of hepatoma was provided.Methods　Tissue culture supported by filter paper-MTT endpoint-computer image analysis were employed in the in vitro drug sensitivity assay.Results　The median inhibition rate (MIR) at 1 time of peak plasma concentration (1 PPC) were 26.5 % for MMC, 13.0% for ADM, 4.0 %～8.5% for DDP, TSPA, FU and VCR. The MIR at 5 PPC were 92.0% for MMC, 41%～50% for ADM, DDP and TSPA, 16.0 %～17.0 % for FU and VCR. MIR of DDP and TSPA at 5 PPC was 5 times more than MIR at 1 PPC. Conclusion　The result of this experiment suggested that the most effect agent was MMC, and effect of DDP and TSPA were relative closely with the dose. Because the relative coefficient (r) between the agents from nature source, such as ADM, MMC and VCR, were larger (r=0.5539～0.7208) at either 1 PPC or 5 PPC, it is shown that some of hepatomas were multidrug resistant.