肾癌组织中Bcl-2和P53蛋白的表达: P53是独立的预后指标

应用抗Bcl-2和抗P53的单克隆抗体，以标准的LSAB方法，分析了113例肾癌标本。结果表明Bcl－2染色阳性为52例（46．0％），但染色结果与临床和病理之间无明显关系、P53阳性者为30例（26．5％）。有淋巴结转移或远处转移的患者，P53染色阳性的比例明显增高（P＜0．01）。P53阳性患者的生存率明显低于阴性患者（P＜0．01），并且是独立的预后因素。Bcl-2和P53蛋白表达之间无相关性。提示Bcl-2免疫染色对肾癌预后无重要预示作用，P53介导肾癌的浸润和转移，P53免疫染色阳性，是肾癌患者生存不利的独立预后指标。

Bcl-2 AND P53 EXPRESSION IN RENAL CELL CARCINOMA: p53 OVEREXPRESSION IS AN INDEPENDENT PROGNOSTIC PARAMETER

To elucidate Bcl 2 and P53 protein expression in renal cell carcinoma(RCC) and their patho clinical significance, the expression of Bcl 2 and P53 proteins was evaluated immunohistochemically and compared with the patho clinical factors from 113 patients. The Bcl 2 positivity was demonstrated in 52 cases(46 0%), but no significant correlation with patho clincal factors could be found. 30 cases (26 5%) were positively stained for P53 protein. P53 overexpression was frequently detected in patients with lymphonode or distal metastases ( P =0 047 and P <0 001, respectively). The overall mortality in renal cancer was significantly related with P53 nuclear overexpression ( P <0 001). It is concluded that Bcl 2 expression will not contribute additional prognostic information. P53 protein is involved in tumor invasion and metastases. P53 immunostaining is of practical value in predicting prognosis of RCC.