初次治疗的肺结核患者血清IL-17检测及意义

目的研究Th17细胞相关因子IL-17在肺结核发病机制中的作用。方法用ELISA法分别测定初治涂阳肺结核患者治疗前组（22例）、治疗1个月组（18例）、治疗6个月组（16例）的IL-17、IFN-y和IL-4水平。以20例健康志愿者为正常对照组。统计分析比较各组间细胞因子水平的差异及相互相关性。结果IL-17、lL-4水平，治疗前组高于治疗1个月组、治疗6个月组及正常对照组（均P〈0．05）；治疗1个月组高于治疗6个月组及正常对照组（均P〈0．05）；而IFN-1水平与前两者恰恰相反。治疗6个月组与正常对照组三者水平差异均无统计学意义（均P〉0．05）。各组中IL-17水平与IFN-y、IL-4水平均无相关性（P〉0．05）。结论人体感染结核杆菌后，IL-17增高。经过抗结核治疗，IL-17水平逐渐降低。提示IL-17在肺结核发病机制中可能起着非常重要的作用。

The level of IL-17 in TB patients post-treatment and its implications

Objective T To study the effect of Interleukin-17 （IL-17）, a Th17 cells related factor, in the pathogenesis of a pulmonary tuberculosis. Methods The levels of IL-17, interferon-y（IFN-y） and interleukin-4 （IL-4） were detemined by enzyme-linked immunosorbent assay （ELISA） in patients with smear-positive pulmonary tuberculosis （22 cases）, 1 month post-treatment （18 cases） and 6 months posttreatment （16 cases）. A total of 20 healthy volunteers were chosen as a control group. The data were analyzed and the significance was implemented with t-test and Pearson correlation analysis. Results Both IL-17 and IL-4 levels were higher in the group before treatment than in any other groups （P〈0.05）. They were also higher in the group one month post-treatment than in the group 6 months post-treatment and the control group （P〈0.05）, However, this was not the case for IFN-y. Moreover, no significant difference in the measured indices was seen between the group 6 months post-treatment and the control group （P〉0.05）. There were no correlation between IL-17 and IFN-y as well as between IL-17 and IL-4 in each group （P〉 0.05）. Conclusion IL-17 levels could be increased after infection with Mycobacterium, and decreased gradually after anti-tuberculosis treatment, suggesting that IL-17 may play a very important role in the pathogenesis of pulmonary tuberculosis.