Single particle adsorbing transfer system |
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Authors: | Daniela Woide Veronika Mayer Thorsten Wachtmeister Norbert Hoehn Albert Zink Udo Koehler Stefan Thalhammer |
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Institution: | 1. Helmholtz Zentrum Munich, German Research Center for Environmental Health (GmbH), Institute of Radiation Protection, Ingolst?dter Landstrasse 1, 85764, Neuherberg, Germany 2. Xyz high precision, Heinestrasse 20, 64295, Darmstadt, Germany 3. EURAC, Institute for the iceman, Viale Druso 1, 39100, Bolzano, Italy 4. Medizinisch Genetisches Zentrum, Bayerstrasse 3-5, 80335, Munich, Germany
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Abstract: | Here we present a novel approach for horizontal transfer of single particles after laser microdissection. The developed technique
is a single particle adsorbing system for highly selective and gentle horizontal transfer of microdissected fixed and living
material. As mediated via low-pressure technology, the transfer process can be precisely controlled, thus facilitating horizontal
particle transfer of any isolated material, e.g. tissue material, single cells or chromosomes, in addition to precise positioning
for sample release. This collection method allows one to predefine target positions and enables material transfer without
contamination to any planar microchip device. This contamination free transfer is indispensable for novel lab-on-a-chip systems
performing nanoscale polymerase chain reaction analyses. Using virtual reaction chamber microdevices, small amounts of microdissected material—as little as one single cell—can be directly transmitted
and immediately used for single cell analysis.
Daniela Woide and Veronika Mayer contributed equally to this paper. |
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Keywords: | Laser microdissection Single particle handling Low pressure LV-PCR microchip |
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