Comparison of agar dilution, broth dilution, disk diffusion, and the E-test for susceptibility testing of penicillin-susceptible and penicillin-resistantStreptococcus pneumoniae |
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| Authors: | J Thorvilson P Kohner N Henry F Cockerill III |
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| Institution: | (1) Division of Clinical Microbiology, Department of Laboratory Medicine, Mayo Clinic and Foundation, Hilton 4, 200 First Street S.W., 55905 Rochester, Minnesota, USA;(2) Department of Pediatrics and Adolescent Medicine, Mayo Clinic and Foundation, Hilton 4, 200 First Street S.W., 55905 Rochester, Minnesota, USA;(3) Division of Infectious Diseases, Department of Internal Medicine, Mayo Clinic and Foundation, Hilton 4, 200 First Street S.W., 55905 Rochester, Minnesota, USA |
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| Abstract: | An evaluation to determine the optimal methods for the in vitro susceptibility testing of 41 clinical isolates and the ATCC 49619 strain ofStreptococcus pneumoniae to penicillin was undertaken. No very major or major interpretive errors were observed with the following test methods and media: agar dilution using either Mueller-Hinton medium with lysed horse blood or Haemophilus test medium; broth dilution using cation-adjusted Mueller-Hinton medium with lysed horse blood, Haemophilus test medium, or Todd-Hewitt medium; and the epsilometer test (E-test) using agar containing Mueller-Hinton medium and 5% sheep blood. The disk diffusion method using agar containing Mueller-Hinton medium and 5% sheep blood agar was an effective screening method, requiring confirmation by a dilution susceptibility test method. |
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