RSV感染哮喘小鼠气道炎症及重构与激素抵抗性的研究

目的观察小鼠哮喘模型感染呼吸道合胞病毒（respiratary syncytial virus，RSV）气道炎症及重构与哮喘治疗激素敏感性之间的联系。方法50只BALB/c小鼠随机分对照组，单纯卵蛋白 (OVA)致敏哮喘组（A组）、OVA致敏哮喘+地塞米松（DEX）组（B组）、OVA致敏哮喘+RSV感染组（C组）、OVA致敏哮喘+RSV感染+ DEX组（D组），每组10只；酶联免疫吸附法（ELISA）检测白细胞介素-4（IL-4）、白细胞介素-5（IL-5）、转化生长因子β₁（TGF-β₁）及γ干扰素（IFN-γ）浓度;病理切片行HE染色、Van Gieson 氏染色，显微镜下观察气管外周炎性分级和气道胶原沉积情况。结果A、C、D组与对照组比较IL-4、IL-5及TGF-β₁浓度升高，气道胶原沉积、炎症浸润加重，差异有统计学意义（P＜0.05）；D组与C组比较，C组与A组比较，TGF-β₁浓度升高，差异有统计学意义（P＜0.05），气道炎症及气道胶原沉积加重。结论RSV感染哮喘小鼠可引起明显的Th2细胞炎性反应及气道重构；应用激素干预治疗后，气道炎症及重构进一步加重，表明RSV感染哮喘小鼠对激素治疗敏感性差。

Correlation between airway inflammation and asthma marine remodeling and corticosteroid resistence in rats with respiratory syncial virus infection

To explore the relationship of airway inflammation and murine model to corticosteroid resistance after respiratory syncytial virus (RSV) infection. Methods 50 BALB/c mice were divided into 5 groups: the control group, the OVA group（group A）, the OVA+Dex group（group B）, the OVA/RSV group(group C), and the OVA/RSV+ Dex group（group D）, 10 mice in each group. Then the concentrations of the interleukin 4（IL-4）, interleukin 5（IL-5）, interferon-γ（IFN-γ）and transforming growth factor-β1(TGF-β₁) were determined by ELISA. Pathological slides were stained with hematoxylin eosin and Van. Gieson, and degree of the peribronchial inflammation and airway collage deposition were observed. ResultsCompared with the control group, concentrations of IL-4, IL-5 and TGF-β₁ were significantly increased in groups A, C and D and airway inflammation and collagen deposition were also aggravated（P＜0.05）. Also concentrations of IL-4, IL-5 and TGF-β₁ and airway inflammation and remodeling were gradually increased in the given order of groups A, C and D（P＜0.05）. Conclusion RSV infection results in a significant Th2 inflammation and airway remodeling in rats with asthma, and the rats are not sensitive to corticosteroid treatment.