| 南京地区耐亚胺培南肠杆菌科细菌碳青霉烯酶及整合子调查 |
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| 引用本文： | 宁明哲,沈瀚,印玉炜,张之烽,周万青,张葵. 南京地区耐亚胺培南肠杆菌科细菌碳青霉烯酶及整合子调查[J]. 中华医院感染学杂志, 2012, 22(19): 4181-4183 |
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| 作者姓名： | 宁明哲 沈瀚 印玉炜 张之烽 周万青 张葵 |
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| 作者单位： | 1. 南京大学医学院附属南京市鼓楼医院检验科,江苏南京,210000
2. 东南大学医学院医学检验系,江苏南京,210008 |
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| 基金项目： | 南京市卫生局(YKK09115) |
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| 摘 要： | 目的 了解南京地区耐亚胺培南肠杆菌科细菌碳青霉烯酶(KPC酶)及整合子分布.方法 收集南京地区3所三级甲等医院共55株耐亚胺培南肠杆菌科细菌,进行改良Hodge试验,PCR扩增检测细菌KPC酶与整合子基因并行序列分析.结果 55株待检菌中,改良Hodge试验阳性39株,阳性率为70.9％；KPC酶PCR扩增阳性39株,阳性率为70.9％；整合子PCR扩增阳性19株,阳性率为34.5％.结论 KPC酶引起的碳青霉烯类耐药需引起临床及实验室的关注.
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| 关 键 词： | 肠杆菌科细菌 碳青霉烯酶 整合子 |
Distribution of KPC enzymes and integrons in imipenem-resistant Enterobacteriaceae in Nanjing |
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| NING Ming-zhe,SHEN Han,YIN Yu-wei,ZHANG Zhi-feng,ZHOU Wan-qing,ZHANG Kui. Distribution of KPC enzymes and integrons in imipenem-resistant Enterobacteriaceae in Nanjing[J]. Chinese Journal of Nosocomiology, 2012, 22(19): 4181-4183 |
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| Authors: | NING Ming-zhe SHEN Han YIN Yu-wei ZHANG Zhi-feng ZHOU Wan-qing ZHANG Kui |
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| Affiliation: | (Nanjing Drum Tower Hospital Affiliated to Medical College, Nanjing University,Nanjing,Jiangsu 210000,China) |
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| Abstract: | OBJECTIVE To investigate the distribution of KPC enzymes and integrons in Enterobacteriaceae resistant to imipenem in Nanjing.METHODS A total of 55 strains of Enterobacteriaceae were collected from 3 tertiary hospitals in Nanjing.The modified Hodge test was performed,KPC was amplified by PCR,and integron genes were sequenced.RESULTS Among 55 strains to be tested,39(70.9%) strains were positive in modified Hodge test;39 strains(70.9%) were positive in PCR amplification of KPC;19 strains(34.5%)were positive in PCR amplification of integrons.CONCLUSION The resistance to carbapenem caused by KPC enzymes should be attached great importance in the clinical laboratores. |
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| Keywords: | Enterobacteriaceae KPC enzymes Integrons |
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