曲美他嗪对大鼠心力衰竭模型心肌氧化相关基因的影响

目的探讨曲美他嗪对大鼠心力衰竭模型心肌氧化相关基因的影响。方法选择SD大鼠24只,随机分为模型组、假手术组、曲美他嗪高剂量组(高剂量组)、曲美他嗪低剂量组(低剂量组),每组6只。采用腹主动脉缩窄法制备SD大鼠心力衰竭模型,电镜观察心肌线粒体结构变化;Real-Time PCR定量分析超氧化物歧化酶(SOD)、血红素氧合酶1、谷胱甘肽过氧化物酶表达;免疫组织化学法检测SOD;放射免疫法检测总抗氧化能力(T-AOC)水平。结果与假手术组比较,模型组、低剂量组、高剂量组(除SOD外)各项指标均明显降低(P<0.05,P<0.01)。与模型组比较,高剂量组、低剂量组线粒体结构明显改善,SOD、T-AOC水平明显升高(P<0.05,P<0.01)。高剂量组SOD表达明显高于假手术组(P<0.05)。结论曲美他嗪能提高大鼠心力衰竭模型心肌各氧化相关基因的表达,改善心功能。

Effect of trimetazidine on expression of myocardial oxidation-related genes in rat heart failure model

Objective To study the effect of trimetazidine(TMZ) on expression of myocardial oxidation -related genes in rat heart failure model.Methods Twenty-four SD rats were randomly divided into model group,sham operation group,high TMZ dose group and low TMZ dose group,6 in each group.A rat heart failure model was established by constricting the abdominal aortic artery. Myocardial mitochondrial structure was observed by electron microscopy.Expressions of SOD,heme oxygenase-1 and glutathione peroxidase were detected by RT-PCR.SOD and total anti -oxidation capability(T-AOC) were assayed by immunohistochemistry and radioimmunoassay, respectively.Results The various indications were significantly lower in model group,low and high TMZ dose groups(except for SOD) than in sham operation group(P<0.05,P<0.01).The mitochondrial structure was much better,and the SOD and T-AOC levels were significantly higher in high and low TMZ dose groups than in model group(P<0.05,P<0.01) while the SOD expression level was significantly higher in high TMZ dose group than in model group(P<0.05). Conclusion TMZ can up-regulate the expression of myocardial oxidation-related genes in rat heart failure model and improve the rat heart function.