钛-6铝-4钒颗粒对破骨细胞形态和功能的影响

目的在体外条件下观察钛-6铝-4钒（Ti-6AL-4V）颗粒对破骨细胞形态和功能的影响。方法取新西兰大耳兔破骨细胞培养在玻璃盖玻片和牛皮质骨片上，实验组用0．1mg／ml颗粒刺激，经不同时段培养后行抗酒石酸酸性磷酸酶（TRAP）染色，观察颗粒对破骨细胞形态的影响。对骨片上形成的骨吸收陷窝进行甲苯胺蓝染色观察，并用计算机图像分析软件评估吸收面积的大小。结果破骨细胞可以吞噬颗粒，形态变得不规则，特征性TRAP染色加深，较早出现凋亡征象。经颗粒刺激后在骨片上形成的骨吸收陷窝面积较大、数量较多。结论破骨细胞有吞噬功能，吞噬Ti-6AL-4V颗粒后发生形态和功能的变化，骨吸收功能增强。

Effect of Ti-6AL-4V particles on morphology and function of osteoclasts: an in vitro study

Objective To observe the effect of Ti-6AL-4V particles on morphological and functional changes of osteoclast in vitro. Methods Mature osteoclasts separated from New Zealand Rabbits were cultured on glass slices and cortical bone slices. The experimental group was stimulated by Ti-6AL- 4V particles at concentration of 0.1 mg/ml. The cells were stained with TRAP at different culture time to observe the morphological variety. The bone resorption pits on bone slices were stained by toluidine blue and the resorption areas analyzed by computer image analysis software. Results Osteoclasts phagocytosed the particles, with irregular shapes, deeper TRAP stain and earlier apoptosis. Stimulation by Ti- 6AL-4V particles brought about larger area of bone absorption lacuna. Conclusion Osteoclasts have the ability to phagocytose Ti-6AL-4V particles, which leads to morphological and functional changes and enhances bone resorption.