对二氯苯致突变性的实验研究

目的　研究对二氯苯的致突变性。方法　Ames试验用TA97、TA98、TA10 0和TA10 2菌株 ,加与不加S 9试验 (剂量设为 0 2 5、0 5、1 0、2 0、5 0mg/皿 ) ;小鼠骨髓多染红细胞微核试验 (剂量设为 10 0、2 0 0、40 0、80 0mg/kg)和小鼠睾丸细胞染色体畸变试验 (剂量设为 2 5 0、5 0 0、10 0 0mg/kg)。结果　Ames试验中各测试浓度的回变菌落数均未超过自然回变菌落数 2倍 ;小鼠骨髓多染红细胞微核试验和小鼠睾丸细胞染色体畸变试验 ,各剂量组和阴性对照组差异无显著性 (P >0 0 5 )。结论　该试验范围内 ,未见对二氯苯有致突变性。

Experimental study on the mutagenicity of P-Dichlorobenzene

To study the mutagenicity of P-Dichlorobenzene.Strains TA97, TA98,TA100 and TA102 with or without S9 were applied to do Ames test (the doses were 0.25, 0.5, 1.0, 2.0 and 5.0 mg/kg). Also applied micronucleus assay of polychromatic erythrocytes (the doses were 100, 200, 400 and 800 mg/kg) and testicle chromosome aberration test on mice (the doses were 250, 550 and 1 000 mg/kg).Ames test showed that P-dichlorobenzene group didn't cause double increase of revertants in every bacterial strain. No significant difference was found in the micronucleus test and the chromosome aberration test.[Conclusion]P-dichlorobenzene has no mutagenic effect according to these tests.