Evaluation of a Modular Multiplex-PCR Methicillin-Resistant Staphylococcus aureus Detection Assay Adapted for mecC Detection |
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| Authors: | Karsten Becker Anders R. Larsen Robert L. Skov Gavin K. Paterson Mark A. Holmes Artur J. Sabat Alexander W. Friedrich Robin K?ck Georg Peters André Kriegeskorte |
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| Affiliation: | Institute of Medical Microbiology, University Hospital Münster, Münster, Germanya;Statens Serum Institute, Copenhagen, Denmarkb;Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdomc;Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlandsd;Institute of Hygiene, University Hospital Münster, Münster, Germanye |
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| Abstract: | A mecC (mecALGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection. |
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