加味茵陈蒿汤影响Nrf2/HO-1信号通路抗大鼠皮肤光老化作用

目的研究加味茵陈蒿汤对UV照射引起的皮肤光老化作用研究,并探讨其作用机制。方法将SD大鼠随机分成4组,随机分为空白组和药物组(加味茵陈蒿汤低浓度组和加味茵陈蒿汤高浓度组)。采用紫外线辐射诱导光老化模型,每天一次,持续10周。光照结束后,通过生化测定各组大鼠皮肤组织中抗氧化酶(CAT、SOD)活性和MDA含量;Western blot法检测p-Nrf2和HO-1蛋白表达。结果给予加味茵陈蒿汤灌药组可明显升高抗氧化酶(CAT、SOD)活力,降低MDA的含量,升高Nrf2/HO-1通路中p-Nrf2和HO-1蛋白表达。结论加味茵陈蒿汤能显著降低光老化模型大鼠氧化应激压力,降低MDA的含量和炎症因子的表达,增加Nrf2/HO-1抗炎途径,促进光老化皮肤的修复,保护皮肤免受紫外线辐射的损害。

Jiawei Yinchenhao Decoction Affects Nrf2/HO-1 Signaling Pathway Against Photoaging of Rat Skin

Objective To study the effect of Jiawei Yinchenhao Decoction on photoaging of skin tissue caused by UV irradiation,and to explore its mechanism of action.Methods SD rats were randomly divided into 5 groups,randomly divided into blank group and drug group (addition of Yinchenhaoshu low concentration group and Jiawei Yinchenhao high concentration group).The photoaging model was induced by ultraviolet radiation once a day for 10 weeks.The activities of antioxidant enzymes (CAT,SOD) and MDA in the skin tissues were determined by biochemical determination.The levels of p-Nrf2 and HO-1 were detected by Western blot.Result The administration of Jiawei Yinchenhao Decoction group can significantly increase the activity of antioxidant enzymes (CAT,SOD),reduce the content of MDA.The expression of p-Nrf2 and HO-1 protein in Nrf2/HO-1 pathway was increased.Conclusion Jiawei Yinchenhao Decoction can significantly reduce the oxidative stress of rats in photoaging model,reduce the content of MDA and the expression of inflammatory factors,promotes the anti-inflammatory pathway of Nrf2/HO-1.Repair of photoaging skin to protect the skin from UV radiation.