| 应用合成肽检测戊型肝炎病毒抗体的实验研究 |
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| 引用本文： | 李顺天,高桂芝,闫志慧,梁树仁,朱理珉,金澍. 应用合成肽检测戊型肝炎病毒抗体的实验研究[J]. 实用肝脏病杂志, 1999, 0(2) |
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| 作者姓名： | 李顺天 高桂芝 闫志慧 梁树仁 朱理珉 金澍 |
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| 作者单位： | 天津市传染病医院科研室 300192(李顺天，高桂芝，闫志慧，梁树仁，朱理珉)，天津市传染病医院科研室 300192(金澍) |
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| 摘 要： | 本文利用多肽合成技术在戊型肝炎病毒(HEV)基因结构区内的开放读码框(ORF)-2和ORF-3区合成了P1、R2二条具有明确抗原表位的多肽,作为EIA法抗-HEV诊断试剂的固相抗原测定抗-HEV。在急性甲型肝炎中抗-HEV的检出率为7.8％,慢性乙型肝炎的检出率为2.9％,志愿献血员1.4％,急性输血性丙型肝炎为0％。抗-HEV诊断试剂检测的CV值为6.8％-9.1％,经162例血清标本的检测与Genelaps抗-HEV诊断试剂的结果对比,其符合率达98.8％。结果表明:本室抗-HEV诊断试剂的灵敏度、特异性检测结果满意,精密度(precision)检测优于部颁标准。
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| 关 键 词： | 戊型肝炎病毒 戊型肝炎病毒抗体 合成肽 酶联免疫吸附法 |
THE DETECTION OF ANTI-HEPATITIS E VIRUS ANTIBODY BY EIA USING SYNTHETIC PEPTIDES |
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| Li Shuntian,Gao Guizhi,Yan Zhihui,et al.. THE DETECTION OF ANTI-HEPATITIS E VIRUS ANTIBODY BY EIA USING SYNTHETIC PEPTIDES[J]. Journal of Clinical Hepatology, 1999, 0(2) |
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| Authors: | Li Shuntian Gao Guizhi Yan Zhihui et al. |
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| Affiliation: | Li Shuntian,Gao Guizhi,Yan Zhihui,et al. Infectious Disease Hospital,Tianjin 300192 |
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| Abstract: | Two polypeptides (P1 and P2) were synthesized-acting as solid-antigen to detect serum anti-HEV by EIA. Among 369 patients,the detection rate of anti HEV in hepatitis A was 7. 8% , in hepatitis B 2.9% ,in blood donor 1.4% and in hepatitis C 0%. The value of CV was 6. 8-9.1%. The coincidental rate of detection with Genelaps was 98.8%. |
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| Keywords: | Hepatitis E Virus Anti-HEV Synthetic Peptids |
| 本文献已被 CNKI 等数据库收录！ |
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