| 混合脐血血浆对植物血凝集素诱导的脐血源性T淋巴细胞增殖、分化的影响 |
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| 引用本文： | 耿微,张福琴,张圣明,张云,周英凡.混合脐血血浆对植物血凝集素诱导的脐血源性T淋巴细胞增殖、分化的影响[J].解剖学杂志,2008,31(4). |
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| 作者姓名： | 耿微 张福琴 张圣明 张云 周英凡 |
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| 作者单位： | 潍坊医学院组织学与胚胎学教研室,潍坊,261042;济宁市精神病防治院二病房,济宁,272051 |
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| 摘 要： | 目的:探讨混合脐血血浆(CBP)在植物血凝集素(PHA)诱导脐血源性T淋巴细胞巾的作用.方法:收集脐血血浆和脐血单个核细胞;将脐血单个核细胞分为CBP PHA和PHA两组.检测两组细胞增殖率及免疫表型.结果:CBP PHA组细胞增殖指数低于PHA组.但无统计学意义;两组细胞表型都以CD3 、CD4 为主,两组差异无统计学意义.结论:混合脐血血浆对脐血源性T淋巴细胞增殖、分化影响不明显,可能与RPMI1640培养体系具有相似的效果.
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| 关 键 词： | 脐血血L浆 T淋巴细胞 增殖 分化 |
Effects of mixed cord blood plasma on proliferation and differentiation of PHA-induced cord blood-derived T lymphocyte |
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| Geng Wei,Zhang Fuqin,Zhang Shengming,Zhang Yun,Zhou Yingfan.Effects of mixed cord blood plasma on proliferation and differentiation of PHA-induced cord blood-derived T lymphocyte[J].Chinese Journal of Anatomy,2008,31(4). |
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| Authors: | Geng Wei Zhang Fuqin Zhang Shengming Zhang Yun Zhou Yingfan |
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| Abstract: | Objective: To explore the effects of mixed cord blood plasma(CBP) on phytohemagglutin(PHA) induction of cord blood-derived T cells.Methods: Cord blood plasma and umbilical cord blood mononuclear cells(CBMCs) were collected;CBMCs were divided two groups: CBP PHA group and PHA group,and the proliferation index and immune phenotypes of each group were detected.Results: The proliferation index of CBP PHA group was lower than that in the PHA group without significant difference(P>0.05);the immune phenotypes of two groups were both mainly CD3 and CD4 without significant difference(P>0.05).Conclusion: The effects of CBP on proliferation and differentiation of cord blood-derived T cells were similar to those of 20% RPMI1640. |
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| Keywords: | cord blood plasma T lymphocyte proliferation differentiation |
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