| Effects of Anthopleurin-Q on Concentration in Cultured the Intracellular Free Ca^2＋ Rat Cortical Astrocytes |
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| 引用本文： | LIU Jue WANG Fang LONG Li-hong LIN Fan-kai YAO Wei-xing WANG Wei CHEN Jian-guo. Effects of Anthopleurin-Q on Concentration in Cultured the Intracellular Free Ca^2＋ Rat Cortical Astrocytes[J]. 神经损伤与功能重建, 2006, 1(1): 5-10 |
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| 作者姓名： | LIU Jue WANG Fang LONG Li-hong LIN Fan-kai YAO Wei-xing WANG Wei CHEN Jian-guo |
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| 作者单位： | [1]Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China [2]Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| 摘 要： | Objective： To investigate the effect of anthopleurin Q （AP Q） on the intracellular Ca^2＋ concentration （ECa^2＋]i） in cultured cortical astrocytes of rats. Methods： The [Ca^2＋]i was monitored by calcium imaging with Ca^2＋ sensitive fluorescent probe fura 2. Results： A concentration of 300 nmol/L AP-Q increased the [Ca^2＋]i in astrocytes by （136.98%＋35.63%） （n=28）,when compared with the baseline level. Furthermore, the elevation of [Ca2＋]i was prevented by extracellular calcium free solution or when the extraeellular Na^＋ was replaced by NMDG^＋ , and was decreased by Ni^＋ ,a non specific antagonist of Na^＋/Ca^2＋ exchanger. Conclusion： AP-Q induced the intracellular [Ca^2＋]i elevation in cultured rat cortical astrocytes via activating the reverse mode of Na^＋/Ca^2＋ exchanger. AP-Q may be a useful tool to develop experimental model of seizures.
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| 关 键 词： | 星形胶质细胞 钙离子 钠离子 离子交换 |
| 文章编号： | 1001-117X（2006）01-0005-06 |
| 收稿时间： | 2006-01-18 |
| 修稿时间： | 2006-01-18 |
Effects of Anthopleurin-Q on the Intracellular Free Ca2+ Concentration in Cultured Rat Cortical Astrocytes |
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| LIU Jue,WANG Fang,LONG Li-hong,LIN Fan-ka,YAO Wei-xing,WANG Wei,CHEN Jian-guo. Effects of Anthopleurin-Q on the Intracellular Free Ca2+ Concentration in Cultured Rat Cortical Astrocytes[J]. Neural Injury and Functional Reconstruction, 2006, 1(1): 5-10 |
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| Authors: | LIU Jue WANG Fang LONG Li-hong LIN Fan-ka YAO Wei-xing WANG Wei CHEN Jian-guo |
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| Abstract: | Objective: To investigate the effect of anthopleurin-Q (AP-Q) on the intracellular Ca2 concentration ([Ca2 ]i) in cultured cortical astrocytes of rats. Methods: The [Ca2 ]i was monitored by calcium imaging with Ca2 sensitive fluorescent probe fura-2. Results: A concentration of 300 nmol/L AP-Q increased the [Ca2 ]i in astrocytes by (136.98%±35.63%) (n=28),when compared with the baseline level. Furthermore, the elevation of [Ca2 ]i was prevented by extracellular calcium free solution or when the extracellular Na was replaced by NMDG , and was decreased by Ni ,a non-specific antagonist of Na /Ca2 exchanger. Conclusion: AP-Q induced the intracellular [Ca2 ]i elevation in cultured rat cortical astrocytes via activating the reverse mode of Na /Ca2 exchanger. AP-Q may be a useful tool to develop experimental model of seizures. |
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| Keywords: | anthopleurin-Q astrocytes calcium imaging Na -Ca2 exchanger seizure |
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