A differential staining technique for chromosome identification and its comparison with fluorescence technique |
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| Authors: | P R CHERNAY N B Kardon L Y Hsu L R Shapiro N G Beratis J Kerr K Hirschhorn |
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| Institution: | Department of Pediatrics, Division of Medical Genetics, Mount Sinai School of Medicine, The City University of New York, New York, New York 10029 and Cytogenetics Laboratory, Letchworth Village, Thiells, New York 10984;Department of Pediatrics, Mount Sinai School of Medicine, City University of New York.;U. S. Public Health Service Postdoctoral Trainee in Human Genetics (HD00210).;Cytogenetics Laboratory, Letchworth Village, Thiells, New York.;Career Scientist of the Health Research Council of the City of New York (1–513). |
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| Abstract: | A cytochemical differential staining technique for localizing repetitive DNA in metaphase preparations and quinacrine fluorescent staining of metaphase preparations were used simultaneously, and the methodology is described. An idiogram for the differential staining technique was developed and a comparison of the idiograms of both techniques revealed that the differential staining technique involves primarily the localization of repetitive DNA at or near the centromere, whereas with the quinacrine fluorescence technique nearly the entire length of the chromosome fluoresces to produce characteristic banding patterns. The combined use of these methods can be a valuable tool in the study of human chromosomes and provides a practical aid in the identification of individual chromosomes and the further definition of their structure. |
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