| BMP12诱导恒河猴骨髓间充质干细胞定向分化为腱细胞 |
| |
| 引用本文： | 王启伟,陈昭烈,朴英杰.BMP12诱导恒河猴骨髓间充质干细胞定向分化为腱细胞[J].解放军医学杂志,2005,30(1):47-50. |
| |
| 作者姓名： | 王启伟 陈昭烈 朴英杰 |
| |
| 作者单位： | [1]InstituteofBiotechnology,AcademyofMilitaryMedicalSciences,Beijing100071 [2]DepartmentofAnatomy,NarfangMedicalUniversity,Guangzhou510515 |
| |
| 基金项目： | NationalKeyBasicResearchProgram (973Program) ,No.G1 9990 5430 8 4 |
| |
| 摘 要： | 目的研究骨髓间充质干细胞定向分化为腱细胞的可能性。方法通过电穿孔法将外源基因BMP12导入骨髓间充质干细胞中，诱导骨髓间充质干细胞定向分化为腱细胞；在形态学和分子生物学水平上对诱导后的细胞加以鉴定。结果光镜下，诱导后的细胞形态发生明显改变；RT-PCR结果表明，诱导后的细胞有BMP12和Collagen Ⅰ的mRNA表达，而没有Coilagen Ⅲ的mRNA表达；诱导后98.39％的细胞呈CD44^ 、HLA-DR^-。结论BMP12能够诱导骨髓间充质干细胞定向分化为腱细胞，间充质干细胞有可能成为肌腱组织工程种子细胞来源之一。
|
| 关 键 词： | 间充质干细胞 骨形态发生蛋白12 定向分化 基因转染 腱细胞 |
The committed differentiation of bone marrow mesenchymal stem cells into tenocytes |
| |
| Wang Qiwei,Chen Zhaolie,PIAO Yingjie.The committed differentiation of bone marrow mesenchymal stem cells into tenocytes[J].Medical Journal of Chinese People's Liberation Army,2005,30(1):47-50. |
| |
| Authors: | Wang Qiwei Chen Zhaolie PIAO Yingjie |
| |
| Institution: | 1. Institute of Biotechnology, Academy of Military Medical Sciences, Beijing,100071
2. Department of Anatomy, Nanfang Medical University, Guangzhou,510515 |
| |
| Abstract: | Objective To investigate the feasibility of committed differentiation of rhesus bone marrow mesenchymal stem cells (MSCs) into tenocytes induced by BMP12. Methods MSCs were transfected with pTARGETTM bearing BMP12 gene by electroporation. The transfected cells were identified by morphological observation and molecular biological measure. Results Under the observation of light microscope, the morphological features of transfected cells changed significantly compared to parental MSCs. RT-PCR data showed the transfected cells had the mRNA expression of BMP12 and collagen Ⅰ, but without that of collagen Ⅲ. 98.39% of the transfected cells were CD44+ and negative for HLA-DR. Conclusion BMP12 could induce MSCs into tenocytes, and bone marrow MSCs might be the optional seed cells for tendon tissue engineering. |
| |
| Keywords: | mesenchymal stem cells bone morphogenetic protein 12 committed differentiation gene transfection tenocytes |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
