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Chloroplast microsatellite markers for the Neotropical orchid genus Epidendrum, and cross-amplification in other Laeliinae species (Orchidaceae) |
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| Authors: | Fábio Pinheiro Clarisse Palma-Silva Fábio de Barros Leonardo P. Félix Christian Lexer Salvatore Cozzolino Mike F. Fay |
| Affiliation: | 1. Departamento de Botanica, Instituto de Biociências, Universidade de S?o Paulo, Rua do Mat?o 277, S?o Paulo, SP, 05508-900, Brazil 2. Jodrell Laboratory, Royal Botanic Gardens Kew, Richmond, Surrey, TW9 3DS, UK 3. Instituto de Botanica, Se??o de Orquidário do Estado, S?o Paulo, SP, 04301-012, Brazil 4. Departamento de Fitotecnia, Laboratório de Citogenética Vegetal, Universidade Federal da Paraíba, Areia, PB, Brazil 5. Department of Biology, Unit of Ecology and Evolution, University of Fribourg, 1700, Fribourg, Switzerland 6. Dipartimento di Biologia Strutturale e Funzionale, Complesso Universitario di Monte S. ?ngelo, Università degli Studi di Napoli Federico II, 80100, Naples, Italy |
| Abstract: | One of the most significant challenges confronting orchid researchers is the lack of specific molecular markers, mainly for species in the Neotropics. Here we report the first set of specific chloroplast microsatellite primers (cpSSR) developed for Neotropical orchids. In total, nine polymorphic cpSSR loci were isolated and characterized in four species occurring in the Brazilian Atlantic Rainforest: Epidendrum cinnabarinum, E. denticulatum, E. fulgens and E. puniceoluteum. Levels of intraspecific polymorphism were characterized using two populations for each species, with 13–20 individuals each. Allele numbers varied from two to three per locus, while the number of haplotypes ranged from three to six per species. Extensive differentiation among the taxa was detected. All markers were successfully cross-amplified in eight other different genera. These cpSSRs markers will enable novel insights into the evolution of this important Neotropical genus. |
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