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| 小胶质细胞体外吞噬模型的建立及相关炎症因子的研究 | |
| 引用本文： | 聂盼,刘勇,杨学森,肖明春,刘堰.小胶质细胞体外吞噬模型的建立及相关炎症因子的研究[J].中国病理生理杂志,2011,27(9):1848-1851. |
| 作者姓名： | 聂盼 刘勇 杨学森 肖明春 刘堰 |
| 作者单位： | 1. 西南大学生命科学学院,重庆 400715; 2. 第三军医大学西南医院眼科,重庆 400038; 3. 第三军医大学军事预防医学院劳动卫生学教研室, 重庆 400038 |
| 基金项目： | 国家自然科学基金资助项目 |
| 摘 要： | 目的: 建立以细胞凋亡信号－磷脂酰丝氨酸(PS)介导的小胶质细胞体外吞噬模型,研究小胶质细胞发挥吞噬功能时炎症因子的表达变化。方法: 用N-乙基马来酰胺(NEM)预处理红细胞,随后整合氧化PS,制备含氧化PS信号的红细胞凋亡模型,并测定小胶质细胞对整合氧化PS信号红细胞吞噬率的变化。同时, real-time PCR检测小胶质细胞中炎症因子白细胞介素1β(IL-1β)和肿瘤坏死因子(TNF-α)的表达情况,研究小胶质细胞吞噬功能与炎症功能之间的关系。结果: 小胶质细胞对整合氧化PS红细胞的吞噬率显著高于对照组(P<0.01),同时炎症因子IL-1β和TNF-α的mRNA表达水平明显减少。结论: 成功制备小胶质细胞体外吞噬凋亡细胞模型,小胶质细胞的吞噬作用可能是自身炎症因子IL-1β和TNF-α在转录水平上降低的诱因。 |
| 关 键 词： | 小神经胶质细胞 磷脂酰丝氨酸类 吞噬作用 细胞因子类 |
| 收稿时间： | 2010-11-15 |
An in vitro phagocytosis model of microglia and factors related to inflammation |
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| NIE Pan,LIU Yong,YANG Xue-sen,XIAO Ming-chun,LIU Yan.An in vitro phagocytosis model of microglia and factors related to inflammation[J].Chinese Journal of Pathophysiology,2011,27(9):1848-1851. | |
| Authors: | NIE Pan LIU Yong YANG Xue-sen XIAO Ming-chun LIU Yan |
| Institution: | 1. School of Life Science, Southwest University, Chongqing 400715, China; 2. Department of Ophthalmology, The Southwest Hospital,Third Military Medical University, Chongqing 400038, China; 3. Department of Occupational Hygiene,College of Military Preventive Medicine, Third Military Medical University, Chongqing 400038, China. |
| Abstract: | AIM: To establish a phagocytosis model of microglia mediated by apoptotic signal phosphatidylserine(PS) and to explore the expression of inflammatory cytokines in the phagocytic process of microglia.METHODS: The apoptosis model of red blood cells(RBC) was established by incubating RBC with oxidized PS(PSox) liposomes after pretreatment of RBC with N-ethylmaleimide(NEM).The phagocytic rate of microglia to RBC integrated with oxidation of PS signal was measured.Real-time PCR was used to detect the expression ... |
| Keywords: | Microglia Phosphatidylserines Phagocytosis Cytokines |
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