|
|||||
|
|
| 1,25-二羟维生素D3抑制被动致敏人气道平滑肌细胞的增殖 | |
| 引用本文： | 宋颖芳,赖国祥,戚好文,吴昌归. 1,25-二羟维生素D3抑制被动致敏人气道平滑肌细胞的增殖[J]. 中国病理生理杂志, 2011, 27(9): 1828-1831. DOI: 10.3969/j.issn.1000-4718.2011.09.033 |
| 作者姓名： | 宋颖芳 赖国祥 戚好文 吴昌归 |
| 作者单位： | 1. 南京军区福州总医院呼吸与危重症医学科,福建 福州 350025; 2. 第四军医大学西京医院呼吸内科, 陕西 西安 710032 |
| 摘 要： | 目的: 检测1,25-二羟维生素D3 对被动致敏的人气道平滑肌细胞(HASMCs)增殖的调节作用,探讨其对哮喘气道重塑的可能作用。方法: 离体培养HASMCs,并将细胞分为对照组、哮喘组及1,25-(OH)2D3组。MTT法检测细胞增殖活力并确定1,25-(OH)2D3最佳作用浓度;用最佳作用浓度刺激HASMCs 48 h后以流式细胞术测定细胞周期,免疫细胞化学染色(SABC法)检测增殖细胞核抗原(PCNA)的表达。结果: 1,25-(OH)2D3在10-9-10-7 mol/L范围内能显著抑制哮喘血清被动致敏的HASMCs增殖(P<0.05),且10-7 mol/L时抑制作用最强;流式细胞术检测显示这一最佳作用浓度的1,25-(OH)2D3能显著抑制被动致敏HASMCs由G0/G1期向S期的转化;此外,1,25-(OH)2D3能显著抑制被动致敏HASMCs中PCNA的表达。结论: 1,25-(OH)2D3能抑制哮喘血清被动致敏的HASMCs增殖,有助于哮喘气道重塑的防治。 |
| 关 键 词： | 哮喘 气道重塑 1 25-二羟维生素D3 气道平滑肌细胞 细胞增殖 |
| 收稿时间： | 2011-02-25 |
Inhibitory effect of 1,25-(OH)2D3 on proliferation of airway smooth muscle cells from passively-sensitized patients |
|
| SONG Ying-fang,LAI Guo-xiang,QI Hao-wen,WU Chang-gui. Inhibitory effect of 1,25-(OH)2D3 on proliferation of airway smooth muscle cells from passively-sensitized patients[J]. Chinese Journal of Pathophysiology, 2011, 27(9): 1828-1831. DOI: 10.3969/j.issn.1000-4718.2011.09.033 | |
| Authors: | SONG Ying-fang LAI Guo-xiang QI Hao-wen WU Chang-gui |
| Affiliation: | 1. Division of Pulmonary and Critical Care Medicine, Fuzhou General Hospital of Nanjing Military Command, Fuzhou 350025, China; 2. Department of Respiratory Disease, Xijing Hospital, The Fourth Military University, Xi'an 710032, China. |
| Abstract: | AIM: To investigate the effects of 1,25-dihydroxyvitamin D3[1,25-(OH)2D3] on the proliferation of passively-sensitized human airway smooth muscle cells(HASMCs),and to explore its potential role in asthmatic airway remodeling.METHODS: HASMCs were passively sensitized with 10% serum from asthmatic patients.1,25-(OH)2D3 was used as the interventor.The effect of 1,25-(OH)2D3 on the cell proliferation and its optimal concentration were determined by MTT colorimetric assay.The cell cycle analysis was performed by... |
| Keywords: | Asthma Airway remodeling 1 25-dihydroxyvitamin D3 Airway smooth muscle cells Cell proliferation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国病理生理杂志》浏览原始摘要信息 | |
| 点击此处可从《中国病理生理杂志》下载免费的PDF全文 | |