| 睾丸组织中的TSP50启动子甲基化差异的研究 |
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| 引用本文： | 茅淑砚,杨明,蒲春雪,吕静静,王悦增.睾丸组织中的TSP50启动子甲基化差异的研究[J].中国免疫学杂志,2011,27(9):796-799. |
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| 作者姓名： | 茅淑砚 杨明 蒲春雪 吕静静 王悦增 |
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| 作者单位： | 1. 吉林大学病理生物学教育部重点实验室,长春,130021
2. 吉林大学第一医院乳腺外科,长春,130021 |
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| 摘 要： | 目的:检测不同组织中TSP50启动子区域的甲基化状态,研究其表达的机制。方法:分离、纯化鼠睾丸中的精原细胞、精母细胞和精子,分别提取人的睾丸组织、Ntera-2细胞、鼠精原细胞、精母细胞和精子的基因组DNA,用重亚硫酸盐处理基因组DNA,PCR扩增人和鼠的TSP50的启动子CpG岛区域,电泳,提纯,连接于质粒中,转导入细胞中,扩增,提取,纯化质粒,测序。结果:人睾丸组织甲基化水平较低,Ntera-2细胞处于较高水平。鼠的精原细胞和精子细胞甲基化水平较高,精母细胞甲基化水平较低。结论:TSP50启动子区域甲基化位点的甲基化水平调控TSP50蛋白的表达。
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| 关 键 词： | TSP50 肿瘤睾丸抗原 甲基化 |
Difference in TSP50 promoter methylation status of testis tissue |
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| MAO Shu-Yan,YANG Ming,PU Chun-Xue,L Jing-Jing,WANG Yue-Zeng.Difference in TSP50 promoter methylation status of testis tissue[J].Chinese Journal of Immunology,2011,27(9):796-799. |
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| Authors: | MAO Shu-Yan YANG Ming PU Chun-Xue L Jing-Jing WANG Yue-Zeng |
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| Institution: | MAO Shu-Yan,YANG Ming,PU Chun-Xue,L(U) Jing-Jing,WANG Yue-Zeng |
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| Abstract: | Objective:To detect the methylation status of the promoter of TSP50 in different tissues and explore its expression mechanism.Methods:To isolate and purify the spermatogonias,spermatocytes and spermatozoa of mouse testis,and extract the genome DNA of human testis tissue,Ntera-2 cells and spermatogonia,spermatocyte,spermatozoa of mouse respectively.Genome DNA was treated with bisulfite,amplificate the CpG region of the promoter of TSP50 of human and mouse by PCR.Electrophoresis,purify and link them into T ve... |
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| Keywords: | TSP50 |
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