|
|||||
|
|
| 白血病Reh、HL-60、K562细胞系RUNX 3 基因P2启动子甲基化状态与RUNX 3 基因表达 | |
| 引用本文： | 李大彩,吴明彩,张根葆,毕富勇. 白血病Reh、HL-60、K562细胞系RUNX 3 基因P2启动子甲基化状态与RUNX 3 基因表达[J]. 中国病理生理杂志, 2011, 27(10): 2017-2020. DOI: 10.3969/j.issn.1000-4718.2011.10.031 |
| 作者姓名： | 李大彩 吴明彩 张根葆 毕富勇 |
| 作者单位： | 1. 皖南医学院肿瘤生化研究室,安徽 芜湖 241002; 2. 皖南医学院病理生理学教研室,安徽 芜湖 241002 |
| 基金项目： | 安徽省高校自然科学基金资助项目 |
| 摘 要： | 目的: 研究人白血病Reh、HL-60与K562细胞系RUNX 3 基因P2启动子甲基化状态及RUNX 3 基因的表达,分析P2启动子甲基化与基因表达之间的关系。方法: 运用甲基化特异性PCR(MSP)和RT-PCR检测Reh、HL-60与K562细胞系RUNX 3 基因P2启动子的甲基化状态及RUNX 3 基因的表达。结果: Reh及K562细胞系RUNX 3 基因P2启动子甲基化特异性扩增呈阳性,非甲基化特异性扩增呈阴性,RT-PCR扩增呈阴性;而HL-60细胞系相反,甲基化特异性扩增呈阴性,而非甲基化特异性扩增呈阳性,RT-PCR扩增阳性。结论: Reh及K562细胞中存在RUNX 3 基因P2启动子的甲基化,且在不同类型白血病细胞系中的甲基化状态不同。 |
| 关 键 词： | 白血病 基因 RUNX3 甲基化特异性PCR |
| 收稿时间： | 2011-04-13 |
Methylation status of RUNX3 gene P2 promoter and expression of RUNX3 in human leukemic cell lines Reh, HL-60 and K562 |
|
| LI Da-cai,WU Ming-cai,ZHANG Gen-bao,BI Fu-yong. Methylation status of RUNX3 gene P2 promoter and expression of RUNX3 in human leukemic cell lines Reh, HL-60 and K562[J]. Chinese Journal of Pathophysiology, 2011, 27(10): 2017-2020. DOI: 10.3969/j.issn.1000-4718.2011.10.031 | |
| Authors: | LI Da-cai WU Ming-cai ZHANG Gen-bao BI Fu-yong |
| Affiliation: | 1. Laboratory of Tumor Biochemistry, Wannan Medical College, Wuhu 241002, China; 2. Department of Pathophysiology, Wannan Medical College, Wuhu 241002, China |
| Abstract: | AIM:To investigate the relationship between the methylation status of RUNX3 gene P2 promoter and expression of RUNX3 in human leukemic cell lines Reh,HL-60 and K562.METHODS: Methylation status of RUNX3 gene P2 promoter was detected by methylation-specific polymerase chain reaction(MSP) and the mRNA expression of RUNX3 was determined by RT-PCR in leukemic cell lines Reh,HL-60 and K562.RESULTS: Methylation-specific PCR products were detected in Reh cells and K562 cells,while those in HL-60 cells were negative... |
| Keywords: | Leukemia Genes RUNX3 Methylation-specific PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《中国病理生理杂志》浏览原始摘要信息 | |
| 点击此处可从《中国病理生理杂志》下载免费的PDF全文 | |