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| Slit2/Robo1信号对鸡胚早期神经管及体节发育的影响 | |
| 引用本文： | 王广,王晓钰,李艳,王丽京,雷健,张笑坛,耿建国,杨雪松. Slit2/Robo1信号对鸡胚早期神经管及体节发育的影响[J]. 中国病理生理杂志, 2011, 27(8): 1592-1596. DOI: 10.3969/j.issn.1000-4718.2011.08.025 |
| 作者姓名： | 王广 王晓钰 李艳 王丽京 雷健 张笑坛 耿建国 杨雪松 |
| 作者单位： | 1. 暨南大学香港大学脑功能与健康联合实验室,暨南大学医学院组织与胚胎学系,广东 广州 510632; 2. 广东药学院血管生物学实验室,广东 广州 510224 |
| 基金项目： | 国家重点基础研究发展计划(973)项目，国家自然科学基金资助项目，中央高校基本科研业务费专项资金资助项目 |
| 摘 要： | 目的: 探讨Slit2/Robo1对鸡胚早期神经管和体节发育的影响。 方法: 显微注射法将质粒注射入HH10期胚胎神经管内,活体胚胎细胞电穿孔方法转染胚胎半侧神经管,以另一侧神经管为对照侧,原位杂交及免疫荧光方法观察转染10 h后神经管的发育和神经嵴细胞迁移至体节的情况。结果: 下调Robo1侧神经管发育较正常对照侧异常,同时发现Slug表达和神经嵴细胞迁移至体节路线发生改变。结论: Slit2/Robo1信号可能通过影响Slug基因表达,对胚胎早期神经管闭合、神经嵴细胞正常产生及迁移方向以及体节分化有重要作用。 |
| 关 键 词： | Slit2/Robo1信号 神经管 神经嵴细胞 体节 生皮肌节 |
| 收稿时间： | 2011-02-20 |
Role of Slit2/Robo1 signaling in development of neural tube and somites in early chick embryos |
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| WANG Guang,WANG Xiao-yu,LI Yan,WANG Li-jing,LEI Jian,ZHANG Xiao-tan,GENG Jian-guo,YANG Xue-song. Role of Slit2/Robo1 signaling in development of neural tube and somites in early chick embryos[J]. Chinese Journal of Pathophysiology, 2011, 27(8): 1592-1596. DOI: 10.3969/j.issn.1000-4718.2011.08.025 | |
| Authors: | WANG Guang WANG Xiao-yu LI Yan WANG Li-jing LEI Jian ZHANG Xiao-tan GENG Jian-guo YANG Xue-song |
| Affiliation: | 1. Joint Laboratory for Brain Function and Health, Jinan University and The University of Hong Kong, Department of Histology and Embryology, School of Medicine, Jinan University, Guangzhou 510632, China; 2. Institute of Vascular Biological Sciences, Guangdong Pharmaceutical University, Guangzhou 510224, China |
| Abstract: | AIM: To investigate the effects of Slit2/Robo1 signaling on the development of neural tube and somites in early chick embryos.METHODS: Plasmid DNA was injected into the lumen of the neural tube from dorsal side of HH10 chick embryo using microinjection,and then in ovo electroporation was performed at half-side of neural tube while another side served as control.Subsequent 10-hour incubation was carried on after transfection until the development of neural tube and neural crest cells migrating to somites wer... |
| Keywords: | Slit2/Robo1 signaling Neural tube Neural crest cells Somites Dermomyotome |
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